Effect of edeine B1 in gene expression of Fusarium graminearum
Ontology highlight
ABSTRACT:
ORGANISM(S): Fusarium graminearum
PROVIDER: GSE214312 | GEO | 2022/10/02
REPOSITORIES: GEO
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Project description:Gene expression of vegetative hyphae from Fusarium graminearum
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Project description:Gene expression of vegetative hyphae from Fusarium graminearum
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Project description:Gene expression from aerial hyphae of Fusarium graminearum
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Project description:Fusarium graminearum (teleomorph Gibberella zeae) is a prominent pathogen that infects major cereal crops, such as wheat, barley, and maize. FSS1 contains a Zn(II)2Cys6 fungal-type DNA-binding domain and localized exclusively to nuclei responding to sodium, suggesting that FSS1 is a TF required for sodium tolerance. By RNA-seq and genetic studies, we found a P-type ATPase pump (FgENA5) that is under control of FSS1 and is responsible for phenotypic defects of fss1 mutants. The wild-type, fss1 deletion, fss1 overexpression mutant strains were incubated in potato dextrose broth (PDB) with or without 1 M NaCl supplementation for an hour. 6 samples examined: 1 h after inoculation of Fusarium graminearum wild-type, Δfss1(Δfss1::gen), and fss1 overexpression mutant (fss1::gen-Pef1a-fss1) strains in potato dextrose broth with or without 1 M NaCl supplementation
2014-07-09 | E-GEOD-59177 | biostudies-arrayexpress
Project description:【Objective】 The objective of this study is to study the transcriptome regulation mechanism of F. graminearum under different pH stress conditions, analyze the gene expression level and its differences, and explore the metabolic pathways related to the anti-stress response of F. graminearum cells under acidic or alkaline conditions, and reveal how F. graminearum actively regulates intracellular metabolism and synthesis processes to adapt to the changes of extracellular pH environment. 【Method】 F. graminearum was cultured in PDB (potato dextrose broth) medium with initial pH of 4.5, 6.5 and 8.0 for 48 h, and the total RNA of the strain was extracted to construct a cDNA library. Transcriptome sequencing and bioinformatics techniques were used to identify the related differentially expressed genes (DEGs), and the metabolic pathways involved were further analyzed. 【Result】 A total of 4283 DEGs were detected under acidic conditions, of which 2232 were up-regulated and 2252 were down-regulated. Under alkaline conditions, there were a total of 498 DEGs, of which 269 were up-regulated and 229 were down-regulated. The results of Gene Ontology (GO) functional enrichment analysis showed that 211 GO terms were significantly enriched and 72 were down-regulated under acidic conditions. There were 33 GO terms that were revised upwards and 40 downwards under alkaline conditions. The results of KEGG encyclopedia of genes and Genomes (Kyoto Encyclopedia of Genes and Genomes) enrichment analysis showed that 22 pathways were significantly enriched and 32 pathways were down-regulated under acidic conditions. There were 8 up-regulated pathways and 13 down-regulated pathways under alkaline conditions. The expression of membrane transporters and hydrolysis of carbohydrate compounds and other related genes were up-regulated, and the expression of genes related to protein metabolism was down-regulated, which assisted F. graminearum cells to adapt to changes in the external environment. At the same time, F. graminearum maintained the internal environment balance of its own cells by reducing secondary metabolism and amino acid metabolism under acidic and alkaline conditions, respectively, so as to resist extracellular pH stress. 【Conclusion】 In the acidic environment, Fusarium graminearum adapts to the changes in the extracellular environment by promoting the production of riboprotein complexes and secondary metabolism. In an alkaline environment, Fusarium graminearum cells respond to and sense external stresses through amino acid metabolism. The analysis of the metabolic pathways of F. graminearum cells provides important gene expression data for the response of F. graminearum to different pH environments, and the results of this study are helpful to understand the pathogenesis of F. graminearum.
2025-05-01 | GSE267311 | GEO
Project description:Purpose: To assess global changes of fusarium graminearum gene expressional responses to different nitrogen source and gene expression changes after removal different histone methylation hallmarks. Methods: For RNA seq of fusarium, three biological replicates of hyphae after 48/72 hours cultured were sequenced. Results: For each sample, 5.83-8.31G RNA-seq clean reads were obtained mapped to the Fusarium graminearum genome sequence.
2025-04-01 | GSE171288 | GEO
Project description:Fusarium graminearum (teleomorph Gibberella zeae) is a prominent pathogen that infects major cereal crops, such as wheat, barley, and maize. FSS1 contains a Zn(II)2Cys6 fungal-type DNA-binding domain and localized exclusively to nuclei responding to sodium, suggesting that FSS1 is a TF required for sodium tolerance. By RNA-seq and genetic studies, we found a P-type ATPase pump (FgENA5) that is under control of FSS1 and is responsible for phenotypic defects of fss1 mutants. The wild-type, fss1 deletion, fss1 overexpression mutant strains were incubated in potato dextrose broth (PDB) with or without 1 M NaCl supplementation for an hour.
2014-07-09 | GSE59177 | GEO
Project description:High throughput phosphoproteomics analysis of F. graminearum DOAM233423
2013-06-19 | PRD000382 | Pride
Project description:The plant pathogenic fungus Fusarium graminearum (Fgr) creates economic and health risks in cereals agriculture. Fgr causes head blight (or scab) of wheat and stalk rot of corn, reducing yield, degrading grain quality and polluting downstream food products with mycotoxins. Fungal plant pathogens must secrete proteases to access nutrition and to breakdown the structural protein component of the plant cell wall. Research into the proteolytic activity of Fgr is hindered by the complex nature of the suite of proteases secreted. We used a systems biology approach comprising genome analysis, transcriptomics and label-free quantitative proteomics to characterise the peptidases deployed by Fgr during growth. A combined analysis of published microarray transcriptome datasets revealed seven transcriptional groupings of peptidases based on in vitro growth, in planta growth, and sporulation behaviours. An orbitrap MS/MS proteomics technique defined the extracellular proteases secreted by Fusarium graminearum.
2015-10-06 | PXD002786 | Pride
Project description:Purpose: To explore the mechanism of Frenolicin B could control the Fusarium Head Blight on Wheat. Methods: Fusarium graminearum were inoculated into PDB broth and cultivated 36 hours. After that, Frenolicin B was added in the broth of Fusarium graminearum, and the same volumes of methanol were served as the control. Then incubated together 6 hours.
2020-03-31 | GSE147731 | GEO