Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

DNA methyltransferase inhibition induces dynamic gene expression changes in lung CD4+ T cells of neonatal mice with E. coli pneumonia

ABSTRACT: This SuperSeries is composed of the SubSeries listed below.

ORGANISM(S): Mus musculus

PROVIDER: GSE214490 | GEO | 2022/12/22

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

DNA methyltransferase inhibition induces dynamic gene expression changes in lung CD4+ T cells of neonatal mice with E. coli pneumonia [RRBS]

Project description:Bacterial pulmonary infections are a major cause of morbidity and mortality in neonates, with less severity in older children (juveniles). Previous studies demonstrated that CD4+ T cells in the mouse lung, whose primary responsibility is coordinating the immune response foreign pathogens, are differentially methylated in neonates compared with juveniles. Nevertheless, the effect of this differential methylation on CD4+ T cell gene expression and response to infection remains unclear. We treated E. coli-infected neonatal (4-day-old) and juvenile (13-day-old) mice with decitabine (DAC), a DNA methyltransferase inhibitor with broad-spectrum DNA demethylating activity, and performed simultaneous genome-wide DNA methylation and transcriptional profiling on lung CD4+ T cells. Juvenile and neonatal mice experienced differential demethylation in response to DAC treatment, with larger methylation differences observed in neonates. By cross-filtering differentially expressed genes between juveniles and neonates with those sites that were demethylated in neonates, we found that interferon-responsive genes such as Ifit1 are the most down-regulated methylation-sensitive genes in neonatal mice. DAC treatment shifted neonatal lung CD4+ T cells toward a gene expression program similar to that of juveniles. Following lung infection with E. coli, lung CD4+ T cells in neonatal mice exhibit epigenetic repression of important host defense pathways, which are activated by inhibition of DNA methyltransferase activity to resemble a more mature profile.

2022-12-22 | GSE214488 | GEO

DNA methyltransferase inhibition induces dynamic gene expression changes in lung CD4+ T cells of neonatal mice with E. coli pneumonia [RNA-Seq]

2022-12-22 | GSE214489 | GEO

Microenvironmental Hypoxia Induces Dynamic Changes in Lung Cancer Synthesis and Secretion of Extracellular Vesicles.

Project description: Not available

| S-EPMC7601203 | biostudies-literature

DNA methylation regulates the neonatal CD4+ T-cell response to pneumonia in mice.

Project description: Not available

| S-EPMC6066312 | biostudies-literature

Dynamic changes in intrathymic ILC populations during murine neonatal development.

Project description: Not available

| S-EPMC6174991 | biostudies-literature

DNA methylation regulates the neonatal CD4+ T-cell response to pneumonia in mice

Project description:This SuperSeries is composed of the SubSeries listed below.

2018-06-04 | GSE106807 | GEO

DNA methylation regulates the neonatal CD4+ T-cell response to pneumonia in mice

Project description:Lung CD4+ T cell responses may determine outcomes in pediatric pnuemonia. We therefore sought to define the transcriptional response and underlying DNA methyaltion landscape of lung CD4+ T cells by treating neonatal (~5 days of age) and juvenile (~15 days of age) mice with 2.8 million CFUs of E. coli or PBS (control) by aspiration. 48 hours later, lung CD4+ T cells were isolated by magnetic bead pre-enrichment and fluorescence-activated cell sorting for RNA-sequencing (RNA-seq) and modified reduced representation bisulfite sequencing (mRRBS).

2018-06-04 | GSE106806 | GEO

DNA methylation regulates the neonatal CD4+ T-cell response to pneumonia in mice

2018-06-04 | GSE106805 | GEO

Neonatal Clonazepam Administration Induces Long-Lasting Changes in Glutamate Receptors.

Project description: Not available

| S-EPMC6193113 | biostudies-literature

Hyperoxic Exposure Caused Lung Lipid Compositional Changes in Neonatal Mice.

Project description: Not available

| S-EPMC7569933 | biostudies-literature