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Time course of the mRNA expression in wild-type Thermus thermophilus HB8 strain grown in a rich medium (labeling:ENZO)

ABSTRACT: In order to observe altered expression profile of wild-type T. thermophilus HB8 strain grown in a rich (TT) medium at 70°C. we analyzed time-dependent mRNA expression profile of the total mRNA from the strain, which corresponds the logarithmic phase to the early stationary phase.

ORGANISM(S): Thermus thermophilus HB8

PROVIDER: GSE21473 | GEO | 2010/10/15

SECONDARY ACCESSION(S): PRJNA129547

REPOSITORIES: GEO

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Time course of the mRNA expression in wild-type Thermus thermophilus HB8 strain grown in a rich medium (labeling:Affymetrix)

Project description:In order to observe altered expression profile of wild-type T. thermophilus HB8 strain grown in a rich (TT) medium at 70°C. we analyzed time-dependent mRNA expression profile of the total mRNA from the strain, which corresponds the logarithmic phase to the early stationary phase.

2010-10-15 | GSE21290 | GEO

Time-course of mRNA expression in wild-type Thermus thermophilus HB8 grown on rich medium_long (labeling:Affymetrix)

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Time course of the mRNA expression in wild-type Thermus thermophilus HB8 strain grown in a rich medium (labeling:ENZO)

Project description:In order to observe altered expression profile of wild-type T. thermophilus HB8 strain grown in a rich (TT) medium at 70Â°C. we analyzed time-dependent mRNA expression profile of the total mRNA from the strain, which corresponds the logarithmic phase to the early stationary phase. Three wild-type T. thermophilus HB8 strains were pre-cultured at 70Â°C for 16 h in 3 ml of TT medium containing 0.8% polypeptone, 0.4% yeast extract, 0.2% NaCl, 0.4 mM CaCl2, and 0.4 mM MgCl2, which was adjusted to pH 7.2 with NaOH. The cells (2 ml) were inoculated into 1 liter of the same medium and then cultivated at 70Â°C. Cells were collected after 180, 240, 300, 360, 420, 480, 540, 600, 680, and 760 min, and then crude RNA was extracted. The expression of each mRNA at each time point was analyzed on a GeneChip.

2010-10-15 | E-GEOD-21473 | biostudies-arrayexpress

Time course of the mRNA expression in wild-type Thermus thermophilus HB8 strain grown in a rich medium (labeling:Affymetrix)

2010-10-15 | E-GEOD-21290 | biostudies-arrayexpress

Expression profile of crp-deficient Thermus thermophilus HB8 strain

Project description:We observed the expression profile of the total mRNA of crp (TTHA1437) deletion mutant of Thermus thermophilus HB8 strain grown in a rich (TT) medium at 70 degC

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Time course of the mRNA expression in wild-type Thermus thermophilus HB8 strain grown on a rich medium (labeling:ENZO)

Project description:We observed the expression profile of the total mRNA of wild-type Thermus thermophilus HB8 strain grown in a rich (TT) medium at 70oC. Keywords: time course

2007-08-05 | GSE7165 | GEO

Time-course of mRNA expression in wild-type Thermus thermophilus HB8 grown on rich medium_long (labeling:Affymetrix)

Project description:In order to observe the growth phase-dependent expression profile of each mRNA of wild-type Thermus thermophilus HB8 strain grown in a rich medium at 70Â°C, we cultured the strain in a medium, and then the expression of total mRNA at the time points corresponding to logarithmic growth phase and early to late stationary growth phases was analyzed by using DNA microarray (GeneChip). Wild-type T. thermophilus HB8 strains were pre-cultured at 70Â°C for 16 h in 3 ml of TT medium containing 0.8% polypeptone, 0.4% yeast extract, 0.2% NaCl, 0.4 mM CaCl2, and 0.4 mM MgCl2, which was adjusted to pH 7.2 with NaOH. The cells (2 ml) were inoculated into 1 liter of the same medium and then cultivated at 70Â°C. Cells were collected after 180, 360, 540, 680, 880, 1000, 1120, and 1240 min, and then crude RNA was extracted. The expression of each mRNA at each time point was analyzed on a GeneChip.

2010-10-15 | E-GEOD-19839 | biostudies-arrayexpress

Comparative expression analysis between TTHA0101-deficient mutant and wild-type of Thermus thermophilus HB8

Project description:We observed the expression profile of the total mRNA of TTHA0101 deletion mutant of Thermus thermophilus HB8 strain grown in a rich (TT) medium at 70°C compared with that of wild type.

2010-10-15 | GSE24184 | GEO

Comparative expression analysis between PfmR (TTHB023) deletion mutant and wild-type of Thermus thermophilus HB8

Project description:We observed the expression profile of the total mRNA of PfmR (TTHB023) deletion mutant of Thermus thermophilus HB8 strain grown in a rich (TT) medium at 70°C compared with that of wild type.

2013-03-18 | GSE36909 | GEO

mRNA expression in wild-type of Thermus thermophilus HB8 strain grown on a rich (TT) medium at 45˚C and 70˚C

Project description:We observed the expression profile of the total mRNA of wild-type of Thermus thermophilus HB8 strain grown in a rich (TT) medium at 45˚C compared with that of 70˚C. Keywords: Growth temperature

2010-08-23 | GSE22962 | GEO

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