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Palmitoylation negatively regulates AEG-1 function

ABSTRACT: In vitro studies revealed that AEG-1 is palmitoylated at cysteine at a.a. 75 position. In order to check how palmitoylation regulates AEG-1 function in vivo we generated a knockin moue using CRISPR/Cas9 taking in which cysteine 75 was mutated to serine (AEG-1-C75S). Hepatocytes were isolated from AEG-1-WT and AEG-1C75S littermates, total RNA was isolated and RNA-sequencing was performed.

ORGANISM(S): Mus musculus

PROVIDER: GSE215113 | GEO | 2023/01/25

REPOSITORIES: GEO

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