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Caco-2 cells: cycling vs 21-days differentiated cells

ABSTRACT: Transcriptional profile of 21-days differentiated Caco-2 cells compared to Caco-2 cycling cells. Goal was to demonstrate the ability of Caco-2 cells to express most of the markers typical of differentiated enterocytes. A novel protocol of line maintenance was used.

ORGANISM(S): Homo sapiens

PROVIDER: GSE21519 | GEO | 2011/04/25

SECONDARY ACCESSION(S): PRJNA125967

REPOSITORIES: GEO

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Caco-2 cells: cycling vs 21-days differentiated cells

Project description:Transcriptional profile of 21-days differentiated Caco-2 cells compared to Caco-2 cycling cells. Goal was to demonstrate the ability of Caco-2 cells to express most of the markers typical of differentiated enterocytes. A novel protocol of line maintenance was used. Two-condition experiment: cycling Caco-2 cells and 21-days differentiated Caco-2 cells. Two biological replicates of each condition in dye-swap configuration

2011-04-25 | E-GEOD-21519 | biostudies-arrayexpress

Caco-2 cells: 21-days differentiated cells maintained with the low density protocol (LD cells) vs 21-days differentiated cells maintained with the high density protocol (HD cells)

2011-04-25 | GSE21518 | GEO

Caco-2 cells: 21-days differentiated cells maintained with the low density protocol (LD cells) vs 21-days differentiated cells maintained with the high density protocol (HD cells)

Project description:Transcriptional profile of 21-days differentiated LD Caco-2 cells compared to 21-days differentiated HD Caco-2 cells. Goal was to find some differences in the genome profile of the LD and HD Caco-2 cells that could be addressed to the different protocols used for the line maintenance. Two-condition experiment: 21-days differentiated Caco-2 cells maintained with the LD protocol and 21-days differentiated Caco-2 cells maintained with the HD protocol. Dye-swap technical replicates.

2011-04-25 | E-GEOD-21518 | biostudies-arrayexpress

Expression data from Caco-2 cells co-cultivated with B. animalis subsp. lactis BB-12

Project description:Although intestinal microbiota play a pivotal role in the development of host immune system this biological issue was not so far studied in great detail. In this study we examined immune response of Caco-2 enterocytes after incubation with common probiotic Bifidobacterium animalis subsp. lactis BB-12 for 4 hours. We used microarrays to inspect the global gene expression of Caco-2 cells upon co-culturing with B. animalis subsp. lactis BB-12 and several distinct immune-related genes up-regulated during this process. One time point (T4) and two controls (T0) were analysed. T0 represent differentiated Caco-2 cells cultivated for 3 weeks. T4 represents differentiated Caco-2 cells cultivated for 3 weeks plus consequent 4 hours of co-cultivation with B. animalis subsp. lactis BB-12. 3 technical replicates for T0-1, T0-2 or T4 were pooled to a single sample, RNA extracted and further used in gene expression experiments.

2010-07-30 | E-GEOD-21930 | biostudies-arrayexpress

Modification of miRNA profile in Caco-2 cells by dietary lipids

Project description:The aim of this study was to detect miRNAs modified by biactive dietary lipids in a cellular model of enterocytes. The selected model was Caco-2 cells Differentiated Caco-2 cells were incubated with micelles containing the lipids for 24 and 48 hours. The expression leveles of the miRNAs were compared with cells treated with empty micelles. Four control and choelsterol samples, three DHA samples and two CLA samples were analyzed variables: Control (Empty micelles), Cholesterol 250 M-BM-5M 48h, DHA 200 M-BM-5M 24h, CLA 200M-BM-5M 24h Biological replicates Control: Samples 137, 159, 160, 210 Cholesterol: Samples 150, 151, 152, 219 DHA: Samples 141, 142, 143 CLA: Samples 144, 146

2015-03-01 | E-GEOD-59153 | biostudies-arrayexpress

Modification of miRNA profile in Caco-2 cells by dietary lipids

Project description:The aim of this study was to detect miRNAs modified by biactive dietary lipids in a cellular model of enterocytes. The selected model was Caco-2 cells

2015-03-01 | GSE59153 | GEO

Caco-2 differentiation

Project description:This is an analysis of Caco-2 BBe cell spontaneous differentiation. JF2dR1-JF2dR4 = proliferating cells; JF8dR1-JF8dR4 = 4 d post-confluent; JF15dR1-JF15dR4 = 11 d pc, differentiated Keywords: time-course

2004-07-29 | GSE1614 | GEO

Expression data from Caco-2 cells co-cultivated with B. animalis subsp. lactis BB-12

2010-07-01 | GSE21930 | GEO

miRNA expression screening in Liver X Receptor (NR1H3) agonist (GW3965) -treated differentiated Caco-2/TC7 cells

Project description:Differentiated human enterocytic Caco-2/TC7 cells grown on transwell were incubated with LXR agonist GW3965 (5µM) or Vehicle DMSO (1:2000 ; vol:vol) for 48 hours. Total RNA were extracted using mirVana miRNA Isolation Kit (life technologies) then processed with Flash Tag Biotin HSR kit (Affymetrix) and hybridized on an Affymetrix GeneChip miRNA 4.0 Array.

2016-06-03 | E-MTAB-3675 | biostudies-arrayexpress

RNA sequencing of enterocytes differentiated from human iPS cells [RNAseq1]

Project description:Our RNA-seq results show the changes in gene expression during differentiation and maturation of iPS-derived enterocytes, as well as that treatment during the last stage with Vitamin D3 (VD3) enhanced enterocyte-related gene expression. Caco-2 and RNA from human adult small intestine were also sequenced as comparison.

2023-05-25 | GSE226661 | GEO

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