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Berberine mitigates 5-fluorouracil-induced intestinal mucosal damage without impairing anti-tumor effect of 5-Fu by regulating the intestinal flora

ABSTRACT: To investigate the mechanism of BBR improved intestinal mucosa induced by 5-fluorouracil-induced in mice

ORGANISM(S): Mus musculus

PROVIDER: GSE215201 | GEO | 2024/08/15

REPOSITORIES: GEO

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Berberine mitigates 5-fluorouracil-induced intestinal mucosal damage without impairing anti-tumor effect of 5-Fu by regulating the intestinal flora

Project description:Berberine mitigates 5-fluorouracil-induced intestinal mucosal damage without impairing anti-tumor effect of 5-Fu by regulating the intestinal flora

| PRJNA889331 | ENA

Berberine protects against hepatocellular carcinoma progression by regulating intrahepatic T cell heterogeneity

Project description:Accumulating evidence suggests that berberine (BBR) exhibits anti-cancer effects in hepatocellular carcinoma (HCC). However, the mechanisms by which BBR regulates the immunological microenvironment in HCC has not been fully elucidated. In this study, we established a mouse model of orthotopic HCC and treated with varying doses of BBR. BBR showed effectiveness in reducing tumor burden in mice with HCC. Cytometry by time-of-flight depicted the alterations in the tumor immune landscape following BBR treatment, revealing the enhancement in the T lymphocytes effector function. In particular, BBR decreased the proportion of TCRbhiPD-1hiCD69+CD27+CD8a+ effector CD8+ T lymphocytes and increased the proportion of Ly6ChiTCRb+CD69+CD27+CD62L+ central memory CD8+ T lymphocytes. Single-cell RNA sequencing further elucidate the effects of BBR on transcriptional profiles of liver immune cell and confirmed the phenotypical heterogeneity of T lymphocytes in HCC immune microenvironment. Additionally, we found that BBR potentially regulated the antitumor immunity in HCC by modulating the receptor-ligand interaction among immune cells mediated by cytokines. In summary, our findings improve the understanding of BBR's impact on protecting against HCC, emphasizing BBR's role in regulating intrahepatic T cell heterogeneity. BBR has the potential to be a promising therapeutic strategy to hinder the advancement of HCC.

2025-05-21 | GSE271243 | GEO

Analysis of gene expression profile in 5-fluorouracil (5-FU)-resistant human colon cancer cells

Project description:We analyzed the differentially regulated genes in 5-fluorouracil-resnstant human colon cancer cells to discover novel biomarkers involved in 5-FU resistance in colorectal cancer.

2017-03-27 | GSE56322 | GEO

EHMT2-mediated chromatin reprogramming in 5-fluorouracil (5-FU) resistant colorectal cancer

Project description:This study generated transcriptomic datasets to investigate the role of EHMT2 in acquired 5-fluorouracil resistance in colorectal cancer models. RNA sequencing was performed in parental and 5-FU resistant HCT116 cell lines under genetic and pharmacological modulation of EHMT2. These datasets support the findings described in the associated manuscript and provide a resource for further investigation of transcriptional mechanisms underlying chemoresistance.

2026-03-13 | GSE314837 | GEO

Berberine attenuates 5-fluorouracil-induced intestinal mucosal injury by modulating the gut microbiota without compromising its anti-tumor efficacy.

Project description: Not available

| S-EPMC11305238 | biostudies-literature

Combined use of astragalus polysaccharide and berberine attenuates insulin resistance in IR-HepG2 cells via regulation of the gluconeogenesis signaling pathway

Project description:Insulin resistance (IR) is likely to induce metabolic syndrome and type 2 diabetes mellitus (T2DM). Gluconeogenesis (GNG) is a complex metabolic process that may result in glucose generation from certain non-carbohydrate substrates. Chinese herbal medicine astragalus polysaccharides and berberine have been documented to ameliorate IR, and combined use of astragalus polysaccharide (AP) and berberine (BBR) are reported to synergistically produce an even better effect. However, what change may occur in the GNG signaling pathway of IR-HepG2 cells in this synergistic effect and whether AP-BBR attenuates IR by regulating the GNG signaling pathway remain unclear. For the first time, we discovered in this study that the optimal time of IR-HepG2 cell model formation was 48 hours after insulin intervention. AP-BBR attenuated IR in HepG2 cells and the optimal concentration was 10mg. AP-BBR reduced the intracellular H2O2 content with no significant effect on apoptosis of IR-HepG2 cells. In addition, a rapid change was observed in intracellular calcium current of the IR-HepG2 cell model, and AP-BBR intervention attenuated this change markedly. The gene sequencing results showed that the GNG signaling pathway was one of the signaling pathways of AP-BBR to attenuate IR in IR-Hepg2 cells. The expression of p-FoxO1Ser256 and PEPCK protein was increased and the expression of GLUT2 protein was decreased significantly in the IR-HepG2 cell model, and both of these effects could be reversed by AP-BBR intervention. AP-BBR attenuated IR in IR-HepG2 cells, probably by regulating the GNG signaling Pathway.

2019-11-14 | GSE139929 | GEO

Berberine Attenuates Intestinal Mucosal Barrier Dysfunction in Type 2 Diabetic Rats.

Project description: Not available

| S-EPMC5290458 | biostudies-literature

Effect of berberine analogues on functional profiles of human gut microbiomes

Project description:The understanding of the effects of compounds on the gut microbiome is limited and in particular we don’t know whether structurally similar compounds have similar or distinct effects on the gut microbiome. Here we selected berberine (BBR), an isoquinoline quaternary alkaloid, and sixteen structural analogues, and evaluated their effects on in vitro cultured individual gut microbiomes. The responses of the individual microbiomes were evaluated by metaproteomic profiles and by assessing butyrate production. BBR and eight analogues led to changes in proteins involved in microbial defense and stress responses, and enrichment of proteins from Verrumicrobia, Proteobacteria and Bacteroides phyla. It also led to a decrease in proteins from the Firmicutes phylum and its Clostridiales order which correlated to decrease proteins involved in the butyrate production pathway and butyrate concentration. Three of the compounds, Sanguinarine, Chelerythrine and Ethoxysanguinarine activated bacterial protective mechanisms, enriched Proteobacteria, increased opacity proteins and markedly reduced butyrate production. Dihydroberberine had a similar function to BBR in enriching the Akkermansia genus. In addition, it showed less overall adverse impacts on the functionality of the gut microbiome, including a better maintenance of the butyrate level. Our study shows that ex vivo microbiome assay can assess differential regulating effects of compounds with subtle differences and reveals that compound analogues can have distinct effects on the microbiome.

2020-07-21 | PXD015934 | Pride

EHMT2-mediated chromatin reprogramming in 5-fluorouracil (5-FU) resistant colorectal cancer

Project description:EHMT2-mediated chromatin reprogramming in 5-fluorouracil (5-FU) resistant colorectal cancer

| PRJNA1392903 | ENA

Global transcriptional profiling of Staphylococcus aureus treated with berberine chloride

Project description:Staphylococcus aureus (S. aureus) is an important human and animal pathogen, multiply resistant strains are increasingly widespread, new agents are needed for the treatment of S. aureus. Berberine chloride (BBR), a natural plant product, has potent antimicrobial activity against S. aureus. We employed Affymetrix Staphylococcus aureus GeneChipsTM arrays to investigate the global transcriptional profiling of Staphylococcus aureus ATCC25923 treated with berberine chloride. Keywords: gene expression array-based, count

2007-05-30 | GSE7944 | GEO

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