Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

ScRNA seq of oral and skin cells from Ikkα or Ikkα/Stat3 inducible ablated mouse with fungi treatment

ABSTRACT: Diverse taxa of fungus kingdom are part of cancer related microbiota but which fungi induce pro-tumor immune responses and whether they impact specific cancer pathways are unclear. Here, we use Cladosporium cladosporioides to orally infect mice lacking Ikka in epithelial stem cells. This infection promotes systemic inflammation, acidic oral milieu with Proteobacteria expansion, and oral and skin squamous cell carcinoma (SCC) development. Double Stat3 and Ikka deletions reverse these phenotypes. We found in the serum of mouse, C. cladosporioides induces IL-17a and Il-1b production. To investigate what is happening in the oral environment as well as in the skin cells of mice, we conducted 10x genomics single cell RNA sequencing on mRNA in oral buccal tissues and skins from Ikka inducible ablated mouse with fungi treatment (Cladosporium cladosporioides).

ORGANISM(S): Mus musculus

PROVIDER: GSE215966 | GEO | 2026/08/20

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

scRNA seq of blood cells from Ikka or Stat3 inducible ablated mouse with fungi treatment

Project description:Diverse taxa of fungus kingdom are part of cancer related microbiota but which fungi induce pro-tumor immune responses and whether they impact specific cancer pathways are unclear. Here, we use Cladosporium cladosporioides to orally infect wildtype mice or mice lacking Ikka or Stat3/Ikka in epithelial stem cells. This infection promotes systemic inflammation, acidic oral milieu with Proteobacteria expansion, and oral and skin squamous cell carcinoma (SCC) development. Double Stat3 and Ikka deletions reverse these phenotypes. We found in the serum of mouse, C. cladosporioides induces IL-17a and Il-1b production. To determine which cells contribute the upregulated level of Il-1b and Il17a in the blood under fungi treament, we conducted 10x genomics single cell RNA sequencing on mRNA in blood cells from WT, Ikka or Stat3/Ikka inducible ablated mice with fungi treatment (Cladosporium cladosporioides).

2026-08-20 | GSE223442 | GEO

scRNA seq of oral and skin cells from Ikkα or Ikkα/Stat3 inducible ablated mouse with fungi treatment

Project description:scRNA seq of oral and skin cells from Ikkα or Ikkα/Stat3 inducible ablated mouse with fungi treatment

| PRJNA891354 | ENA

Expression data from thymic epithelial cells of 5-6 weeks old C57BL/6J mice.

Project description:Defects in thymic epithelial cell development causes autoimmune diseases due to breakdown of central tolerance, including skin inflammation. IKKa may be essential to link central tolerance to skin inflammation. We used microarrays to examine the global gene expression with or without kinase inactive IKKa, and identified a distinct set of down-regulated genes in the presence of kinase-dead IKKa in thymic epithelial cells.

2017-04-01 | GSE52137 | GEO

Distinct Roles of IL-4, IL-13, and IL-22 in Human Skin Barrier Dysfunction and Atopic Dermatitis

Project description:Atopic dermatitis (AD) is a chronic type-2 inflammatory skin disease characterized by epithelial barrier dysfunction. IL-4, IL-13, and IL-22 represent major cytokines contributing to AD-pathogenesis. Models using reconstructed keratinocytes have been used in previous studies, however, did not represent the real skin situation. Here, we report the distinct effects of IL-4, IL-13, and IL-22 on bio-stabilized human skins with intact barriers and resident immune cells. Spatial transcriptomics on AD-lesions and non-lesional skin of patients, and RNA-sequencing, and proteomics were performed in IL-4-, IL-13- and IL-22-treated native skins. Skin barrier integrity was evaluated using electrical impedance spectroscopy. Spatial transcriptomics demonstrated that CLDN1, FLG, and FLG2 were significantly downregulated in the epidermis of AD lesions. IL-4, IL-13, and IL-22 disrupted the skin barrier in the ex vivo human skin by downregulation of the genes and proteins critical for barrier function and keratinization. IL-4 and IL-13 suppressed, whereas IL-22 upregulated the antimicrobial peptides. Interestingly, IL-4 and IL-13 showed a cross-regulation with IL-22, by downregulation of IL-22Rα1 with IL-4 and IL-13 and upregulation of IL-4Rα with IL-22. Three months of dupilumab treatment in AD patients restored the IL-4 and IL-13-dysregulated genes, whereas genes co-regulated or only regulated by IL-22 remained unaltered. In conclusion, this study comprehensively provides insights into the distinct immune profiles following IL-4, IL-13, and IL-22 stimulation in human skin, highlighting their complex interplay in the pathogenesis of AD modulating keratinocyte and immune responses.

2025-12-05 | GSE292848 | GEO

RNAseq of Cladosporium europaeum in presence of PCB congeners

Project description:Polychlorobiphenyls (PCB) are organochlorinated xenobiotics and pollute environmental matrices worldwide. Ascomycetes fungi colonize polluted soils and can transform PCB. Nonetheless, the mechanisms involved in PCB transformation by Ascomycetes fungi are still underexplored. In this study, the Ascomycetes Cladosporium europaeum F32, isolated from a soil historically polluted with PCB, was grown in presence of 4 different PCB congeners to assess its transcriptomic response. To the authors’ knowledge, this is the first in-depth transcriptomic analysis on Ascomycetes fungi growing in presence of PCB congeners.

2026-05-12 | GSE330673 | GEO

Single cell analysis of CD1a-restricted Group A streptococcus (GAS)-reactive peripheral and cutaneous T cells

Project description:Skin and blood single cell RNA sequencing datasets of IL-22- and IFNγ-secreting CD1a-reactive GAS-responsive T cells were generated from four healthy skins, five healthy PBMCs and three psoriatic PBMCs. Using cellular indexing of transcriptomes and epitopes by sequencing (CITE-seq), we measured T cell specific functional states at both protein and transcriptome levels.

2023-07-11 | GSE206326 | GEO

Proteomic study of in vitro epidermis of reconstructed skins made with cells from African or Caucasian skin type

Project description:This study aimed to understand the in vitro behaviour of epidermal cells of African and Caucasian skin types in the context of 3D reconstructed skin. Reconstructed skins epidermis made with cells isolated from skin of African or Caucasian skin type exhibited high differences in stratification and differentiation. The objective of this study is a first global approach to identify at the protein level the differences between reconstructed skins.

| MSV000086147 | MassIVE

scRNA seq of blood cells from Ikka or Stat3 inducible ablated mouse with fungi treatment

Project description:scRNA seq of blood cells from Ikka or Stat3 inducible ablated mouse with fungi treatment

| PRJNA926327 | ENA

Real-time quantitative PCR analysis of 88 microRNAs involved in human cell differentiation and development in involved skin of systemic scleroderma (SSc), localized scleroderma (LSc) and Keloid.

Project description:Skin specimens were derived from involved skin of 3 systemic scleroderma (SSc), 3 localized scleroderma (LSc) and 3 keloid patients. These skin samples and 3 control skins were collected and fixed in formaldehyde immediately after resections. The microRNA (miRNA) isolation from human skin tissue was performed using miRNeasy FFPE kit (Qiagen). For PCR array, miRNAs were reverse-transcribed into first strand cDNA using RT2 miRNA First Strand Kit (SABiosciences). A mixture of equal amounts of miRNAs from 3 normal skins, 3 SSc, 3 LSc or 3 keloid were prepared, and miRNA expression profile in each disease in vivo was evaluated using RT2 Profiler PCR Array. The cDNA was mixed with RT2 SYBR Green/ROX qPCR Master Mix and the mixture was added into 96-well RT2 miRNA PCR Array that includes primer pairs for 88 human miRNAs (SABiosciences). Skin specimens were derived from involved skin of 3 systemic scleroderma (SSc), 3 localized scleroderma (LSc) and 3 keloid patients. These skin samples and 3 control skins were collected and fixed in formaldehyde immediately after resections. Control donors were each matched with diseases for age, sex, and biopsy site.

2013-01-15 | E-GEOD-43469 | biostudies-arrayexpress

Project description:Genome of five Cladosporium fungi

| PRJNA1166724 | ENA