Gene expression in Drosophila melanogaster in response to bacterial infection
Ontology highlight
ABSTRACT:
ORGANISM(S): Drosophila melanogaster
PROVIDER: GSE216042 | GEO | 2023/10/31
REPOSITORIES: GEO
Similar Datasets
Project description:Transcriptome profiling of immune-responsive genes in Drosophila melanogaster during Gram-positive bacterial infection was performed. The most highly induced gene by Micrococcus luteus infection was a novel gene CG44404, named Induced by Infection (IBIN). In the Flybase release FB2019_4 (22.8.2019) IBIN is annotated as coding for a short peptide. In the transcriptome analysis, fourteen immune-inducible long non-coding RNA (lncRNA) genes (Fold Change > 3) were also found.
2019-01-01 | GSE120387 | GEO
Project description:miRNA expression in adult flies infected with the Gram-positive bacteria Micrococcus luteus or the fungi Beauveria bassiana.
2022-07-04 | GSE133862 | GEO
Project description:Transcriptome profiling of immune-responsive genes in Drosophila melanogaster during Gram-positive bacterial infection and upon silencing of osa was performed. The most highly induced gene in the control set (150071 Valanne I) by Micrococcus luteus infection was CR44404, and its expression is fully dependent on osa expression (as shown in this dataset, 150071 Valanne II). The full transcriptome analysis also indicated that Osa regulates the expression of immune genes by modifying the expression of the Drosophila NF-kappaB factors Relish and Dif.
2020-02-13 | GSE120443 | GEO
Project description:Background: The mosquito Anopheles gambiae is a major vector of human malaria. Increasing evidence indicates that blood cells (hemocytes) comprise an essential arm of the mosquito innate immune response against both bacteria and malaria parasites. To further characterize the role of hemocytes in mosquito immunity, we undertook the first genome-wide transcriptomic analyses of adult female An. gambiae hemocytes following infection by two species of bacteria and a malaria parasite. Results: We identified 4047 genes expressed in hemocytes, using An. gambiae genome-wide microarrays. While 279 transcripts were significantly enriched in hemocytes relative to whole adult female mosquitoes, 959 transcripts exhibited immune challenge-related regulation. The global transcriptomic responses of hemocytes to challenge with different species of bacteria and/or different stages of malaria parasite infection revealed discrete, minimally overlapping, pathogen-specific signatures of infection-responsive gene expression; 105 of these represented putative immunity-related genes including anti-Plasmodium factors. Of particular interest was the specific co-regulation of various members of the Imd and JNK immune signaling pathways during malaria parasite invasion of the mosquito midgut epithelium. Conclusion: Our genome-wide transcriptomic analysis of adult mosquito hemocytes reveals pathogen-specific signatures of gene regulation and identifies several novel candidate genes for future functional studies. In order to identify hemocyte-specific and immune-responsive transcripts, we first compared transcripts expressed in hemocytes from one day old sugar-fed mosquitoes to transcripts detected in whole mosquitoes of the same age and feeding status. This resulted in identification of the hemocyte-enriched transcriptome. We then compared hemocytes from 1 day old mosquitoes, 1 hour after immune challenge with heat-killed Escherichia coli or Micrococcus luteus, to control female mosquitoes injected with sterile PBS to determine the bacteria challenge responsive transcriptomes. We used heat-killed bacteria in these assays, because our primary interest was in identifying the bacterial responsive transcriptome and to avoid the potentially confounding effects of altered gene expression due to the lethal effects of a systemic infection associated with injection of living bacteria. Lastly, we compared hemocytes from mosquitoes at 24 hours and 19 days after ingestion of a blood meal infected with Plasmodium berghei to mosquitoes of the same age fed a non-infected blood meal to determine the ookinete and sporozoite infection responsive transcriptomes, respectively. This design resulted in a total of five experimental treatments. The following samples are not included in this submission: Hemo E coli vs. hemo unchallenged A Hemo E coli vs. hemo unchallenged B Hemo m luteus vs. hemo unchallenged A Hemo m luteus vs. hemo unchallenged B
2011-10-31 | E-GEOD-33269 | biostudies-arrayexpress
Project description:Space travel presents unlimited opportunities for exploration and discovery, but requires a more complete understanding of the immunological consequences of long-term exposure to the conditions of spaceflight. To understand these consequences better and to contribute to design of effective countermeasures, we used the Drosophila model to compare innate immune responses to bacteria and fungi in flies that were either raised on earth or in outer space aboard the NASA Space Shuttle Discovery (STS-121). Microarrays were used to characterize changes in gene expression that occur in response to infection by bacteria and fungus in drosophila that were either hatched and raised in outer space (microgravity) or on earth (normal gravity). Whole Oregon R strain drosophila melanogaster fruit flies either raised on earth or in space that were (1) uninfected, (2) infected with bacteria (Escherichia coli), or (3) infected with fungus (Beauveria bassiana) were used for RNA extraction and hybridization on Affymetrix microarrays.
2013-12-11 | E-GEOD-53196 | biostudies-arrayexpress
Project description:Isolated intestinal bacteria from Drosophila melanogaster
| PRJNA306257 | ENA
Project description: Not available
| S-EPMC6812774 | biostudies-literature
Project description:Given the continued advances in mass spectrometry technology and methods for database searching since the previous characterization of the Drosophila melanogaster sperm proteome, a new proteomic analysis of sperm samples was conducted to expand the size and coverage of the sperm proteome. This dataset is part of a larger project examining the molecular life history of Drosophila sperm.
2022-03-14 | PXD027808 | Pride
Project description:Destruxins are a class of insecticidal, anti-viral and phytotoxic cyclic depsipeptides that are also studied for their toxicity to cancer cells. They are produced by various fungi and a direct relationship has been established between Destruxin production and the virulence of the entomopathogen Metarhizium anisopliae. Aside from opening calcium channels, their in vivo mode of action during pathogenesis remains largely uncharacterized. To better understand the effects of a destruxin, we looked at changes in gene expression following injection of Destruxin A into the fruit fly Drosophila melanogaster. Microarray results revealed reduced expression of various antimicrobial peptides (AMPs) that play a major role in the fly’s humoral immune response. Flies co-injected with a non-lethal dose of Destruxin A and the normally innocuous Gram-negative bacteria Escherichia coli, showed increased mortality and an accompanying increase in bacterial titers. Mortality due to sepsis was rescued through ectopic activation of components in the IMD pathway, one of two signal transduction pathways that are responsible for AMP induction. These results demonstrate a novel role for Destruxin A in specific suppression of the humoral immune response in insects. Keywords: Gene Expression
2007-02-10 | GSE5767 | GEO
Project description:This micro-array helps establishing the function of Akirin, a nuclear protein with unknown domains, a putative interacting partner, in the transcriptional regulation of the targets of Relish, a NF-kB factor required to fight Gram(-) bacteria infection in Drosophila melanogaster.
2014-11-05 | GSE54915 | GEO