Project description:We isolated whole livers from male TDG knockout livers (4-month post tamoxifen injection) and HCC, along with their age matched male control livers. RNA was extracted from the samples as per RNAzol manufacturer guidelines with DNase treatment. RNA was then analyzed by bioanalyzer and subjected to sequencing using the Illumina NextSeq 500 platform.
Project description:Inflammation is characterized by a biphasic cycle consisting initially of a pro-inflammatory phase which is subsequently resolved by anti-inflammatory processes. The coordination of these two disparate states needs to be highly controlled, suggesting that the regulation of the cytokines that drive these processes are intimately linked. Interleukin-1 beta (IL1B) is a master regulator of pro-inflammation and is encoded within the same topologically associated domain (TAD) as interleukin-37 (IL37). IL37 has recently emerged as a powerful anti-inflammatory cytokine which diametrically opposes the function of IL1B. Within this TAD, we identified a novel long non-coding RNA called AMANZI which negatively regulates IL1B expression and trained immunity through the induction of IL37 transcription. We found that the activation of IL37 occurs through the formation of a dynamic long-range chromatin contact that leads to the temporal delay of anti-inflammatory responses. The common variant rs16944 present in AMANZI augments this regulatory circuit, predisposing individuals to enhanced pro-inflammation or immunosuppression. Our work illuminates a chromatin-mediated biphasic circuit coordinating expression of IL1B and IL37, thereby regulating two functionally opposed states of inflammation from within a single TAD.
Project description:We have previously shown that Il1a-knockout (KO) mice exhibit rapid (at day 1) and persistent improvements in locomotion associated with reduced lesion volume compared with Il1b-KO mice and C57BL/6 controls after traumatic spinal cord injury (SCI). To investigate the mechanism by which Il1a mediates its detrimental effect, we analyzed the transcriptome of the injured spinal cord of Il1a-KO, Il1b-KO and C57BL/6 mice at 24 hours after SCI using GeneChip microarrays. Il1a-KO, Il1b-KO and C57BL/6 mice were subjected to a 50-kdyn SCI and a 6-mm spinal cord segment centered over the site of contusion extracted for RNA isolation and microarray analysis.
Project description:Livers from C57Bl6 male adult mice infected either with an adenovirus control (Ad-CTRL) or an adenovirus overexpressing FAT10 (Ad-FAT10) and treated or not with pemafibrate (Pema, vehicule CMC) were used to prepare RNA.
Project description:This network was obtained by combining (with an OR logical operator) the following list of IL1B_secretion activators:
TXNIP AND IL1B - reference in pubmed:23747245
IL1B AND hydrogen_peroxide - reference in pubmed:20023662
NLRP3_PYCARD_CASP1_complex AND IL1B AND cholesterol_crystal - reference in pubmed:23747245
IL1B AND NLRP3 AND hydrogen_peroxide - reference in pubmed:20023662
IL1B AND oxLDL AND TLR4 - reference in pubmed:23812099
IL1B AND glucose - reference in pubmed:20023662
IL1B AND IL1B_secretion - reference in pubmed:23747245
IL1B AND CASP8 - reference in pubmed:22365665
NLRP3_PYCARD_CASP1_complex AND NFE2L2 AND IL1B - reference in pubmed:23747245
PYCARD AND IL1B AND hydrogen_peroxide - reference in pubmed:20023662
IL1B AND NLRP3 AND glucose - reference in pubmed:20023662
NFE2L2 AND IL1B - reference in pubmed:23747245
IL1B AND mitochondrial_respiratory_chain_complex_I - reference in pubmed:21124315
TXNIP AND IL1B AND glucose - reference in pubmed:20023662
IL1B AND oxLDL AND CD36 - reference in pubmed:23812099
IL1B AND ripoptosome - reference in pubmed:22365665
NLRP3_PYCARD_CASP1_complex AND IL1B - reference in pubmed:23747245
Project description:Ire1α conditional null or control mice of 3-months old were injected intraperitoneally with TM or vehicle. At 8 hours after the injection, total RNA was isolated from murine liver tissue and subjected to Affymetrix microarray analysis. We used microarrays to profile the global programme of gene expression in the livers of Ire1α null and control mice in the absence or presence of ER stress.
Project description:To explore the effect of IL1B on the transcriptional level of PTC cells, we constructed a PTC cell line with overexpression of the IL1B gene through lentiviral stabilization technology. Subsequently, transcriptome sequencing (RNA-seq) analysis was performed on the cells of the control group and the IL1B overexpression group.
Project description:The Ayu21-18 mouse line (B6;CBA-Bnc2Gt(pU21)18Imeg/Orl; Infrafrontier EM-05043) was used to generate mice with heterozygous inactivation of Bnc2,which were compared to their wild-type (WT) littermates. Mice were fed a pro-fibrogenic NASH diet for 7.5 weeks and livers were used used to prepare RNA.