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Transcriptomic analysis in human 3D skin model injected with resorbable hyaluronic acid fillers reveals foreign body response.

ABSTRACT: This SuperSeries is composed of the SubSeries listed below.

ORGANISM(S): Homo sapiens

PROVIDER: GSE216279 | GEO | 2022/11/16

REPOSITORIES: GEO

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Transcriptomic Analysis in Human 3D Skin Model Injected with Resorbable Hyaluronic Acid Fillers Reveals Foreign Body Response.

Project description:Usage of injectable dermal fillers applied for aesthetic purposes has extensively increased over the years. As such, the number of related adverse reactions has increased, including patients showing severe complications such as product migration, topical swelling and inflammatory reactions of the skin. In order to understand the underlying molecular events of these adverse reactions we performed a genome-wide gene expression study on the multi-cell type human Phenion® Full-Thickness Skin Model exposed to five experimental hyaluronic acid (HA) preparations with increasing cross-linking degree, four commercial fillers from Perfectha®, and non-resorbable filler Bio-Alcamid®. In addition, we evaluated whether cross-linking degree or particle size of the HA-based fillers could be associated with the occurrence of adverse effects. In all cases, exposure to different HA fillers resulted in a clearly elevated gene expression of cytokines and chemokines related to acute inflammation as part of the foreign body response. Furthermore, for one experimental filler genes of OXPHOS complexes I-V were significantly down-regulated (adjusted p-value < 0.05), resulting in mitochondrial dysfunction which can be linked to over-expression of pro-inflammatory cytokines TNFα and IL-1β and chemokine CCL2. Our hypothesis that cross-linking degree or particle size of the HA-based fillers is related to the biological responses induced by these fillers could only partially be confirmed for particle size. In conclusion, our innovative approach resulted in gene expression changes from a human 3D skin model exposed to dermal fillers that mechanistically substantiate aforementioned adverse reactions, and thereby adds to the weight of evidence that these fillers may induce inflammatory and fibrotic responses.

| S-EPMC9654658 | biostudies-literature

Transcriptomic analysis in human 3D skin model injected with resorbable hyaluronic acid fillers reveals foreign body response [RIVM]

Project description:To unravel the molecular events caused by exposure to dermal fillers in a multi-cell type human 3D skin model and to link these events to the characteristics of the dermal fill-ers.

2022-11-16 | GSE216169 | GEO

Transcriptomic analysis in human 3D skin model injected with resorbable hyaluronic acid fillers reveals foreign body response [Perfectha]

2022-11-16 | GSE216167 | GEO

Transcriptomic analysis in human 3D skin model injected with resorbable hyaluronic acid fillers reveals foreign body response.

Project description:Transcriptomic analysis in human 3D skin model injected with resorbable hyaluronic acid fillers reveals foreign body response.

| PRJNA892921 | ENA

Transcriptomic analysis in human 3D skin model injected with resorbable hyaluronic acid fillers reveals foreign body response [RIVM]

Project description:Transcriptomic analysis in human 3D skin model injected with resorbable hyaluronic acid fillers reveals foreign body response [RIVM]

| PRJNA892479 | ENA

Transcriptomic analysis in human 3D skin model injected with resorbable hyaluronic acid fillers reveals foreign body response [Perfectha]

Project description:Transcriptomic analysis in human 3D skin model injected with resorbable hyaluronic acid fillers reveals foreign body response [Perfectha]

| PRJNA892481 | ENA

Modulation of Methacrylated Hyaluronic Acid Hydrogels Enables Their Use as 3D Cultured Model.

Project description:Bioengineered hydrogels represent physiologically relevant platforms for cell behaviour studies in the tissue engineering and regenerative medicine fields, as well as in in vitro disease models. Hyaluronic acid (HA) is an ideal platform since it is a natural biocompatible polymer that is widely used to study cellular crosstalk, cell adhesion and cell proliferation, and is one of the major components of the extracellular matrix (ECM). We synthesised chemically modified HA with photo-crosslinkable methacrylated groups (HA-MA) in aqueous solutions and in strictly monitored pH and temperature conditions to obtain hydrogels with controlled bulk properties. The physical and chemical properties of the different HA-MA hydrogels were investigated via rheological studies, mechanical testing and scanning electron microscopy (SEM) imaging, which allowed us to determine the optimal biomechanical properties and develop a biocompatible scaffold. The morphological evolution processes and proliferation rates of glioblastoma cells (U251-MG) cultured on HA-MA surfaces were evaluated by comparing 2D structures with 3D structures, showing that the change in dimensionality impacted cell functions and interactions. The cell viability assays and evaluation of mitochondrial metabolism showed that the hydrogels did not interfere with cell survival. In addition, morphological studies provided evidence of cell-matrix interactions that promoted cell budding from the spheroids and the invasiveness in the surrounding environment.

| S-EPMC10606912 | biostudies-literature

Fish Gelatin-Hyaluronic Acid Scaffold for Construction of an Artificial Three-Dimensional Skin Model.

Project description:Artificial three-dimensional (3D) skin models have been used as an alternative tool for toxicity testing, skin disease studying, and skin tissue engineering. The 3D skin model can be fabricated using a porous scaffold that provides 3D cellular construction that supports cell attachment and promotes nutrient and air permeation. In this study, fish gelatin (FG) and hyaluronic acid (HA) were selected for scaffold fabrication because they carry no risk of zoonotic disease transmission and are major components of the extracellular matrix (ECM), which may functionally mimic the ECM of native human skin. The FG-HA scaffolds prepared by using a freeze-drying technique were characterized for their porosity, swelling ratio, and mechanical properties. The scaffolds were seeded with dermal fibroblasts and epidermal keratinocytes followed by culturing in air-liquid interface conditions to allow for cell differentiation to form the dermis and epidermis layer, respectively. Histological analysis of the fabricated 3D skin using the FG-HA scaffold clearly exhibited a bilayer of the dermis and epidermis. Additionally, immunochemical staining of the epidermis layer demonstrated the expression of keratin 5, loricrin, and filaggrin, confirming the proliferation and differentiation of keratinocytes. This research evidently suggests that the FG-HA porous scaffold can serve as a potential material for constructing a 3D skin model with characteristics that closely resemble native human skin.

| S-EPMC11886714 | biostudies-literature

Repeated Full-Face Aesthetic Combination Treatment With AbobotulinumtoxinA, Hyaluronic Acid Filler, and Skin-Boosting Hyaluronic Acid After Monotherapy With AbobotulinumtoxinA or Hyaluronic Acid Filler.

Project description:BackgroundFull-face aesthetic treatment involving several treatment modalities may improve facial aesthetic outcome.ObjectiveTo evaluate clinical outcomes and patient perceptions of monotherapy with either abobotulinumtoxinA (ABO) or hyaluronic acid (HA) filler followed by full-face combination treatments of ABO, HA filler, and skin-boosting HA (RSB).Materials and methodsSubjects aged 35 to 50 years were randomized to monotherapy with 50 s.U ABO in the glabella or ≤1 mL HA filler in the nasolabial folds (NLFs)/cheeks. At Month 6 and Month 12, all subjects received combination treatment with ≤50 s.U ABO in the glabella, ≤2 mL HA filler in the NLFs/cheeks (and other facial areas as applicable), and ≤1 mL RSB (additional RSB treatment at Month 7). Assessments included global facial aesthetic appearance and improvement, first impression, perceived age, wrinkle severity, satisfaction questionnaires, and adverse events.ResultsRepeated full-face combination treatment with ABO, HA filler, and RSB was associated with considerably higher levels of aesthetic improvement and subject satisfaction than monotherapy with ABO or HA filler. Improvement rate of glabellar lines was increasing with each treatment.ConclusionRepeated combination treatment achieved greater change in global facial aesthetic appearance than monotherapy. Aesthetic improvement and subject satisfaction was high and increased with each treatment. All treatments were well tolerated.

| S-EPMC7147415 | biostudies-literature

Korean Amberjack Skin-Inspired Hyaluronic Acid Bioink for Reconstruction of Human Skin.

Project description:Decellularized extracellular matrix (dECM) has been extensively employed as tissue engineering scaffolds because its components can greatly enhance the migration and proliferation of cultivating cells. In this study, we decellularized Korean amberjack skin and incorporated soluble fractions in hyaluronic acid hydrogels with 3D-printed tissue engineering hydrogels to overcome any limitation of animal-derived dECM. The hydrolyzed fish-dECM was mixed with methacrylated hyaluronic acid and chemically crosslinked to 3D-printed fish-dECM hydrogels, where fish-dECM contents affected both printability and injectability of the hydrogels. Swelling ratios and mass erosion of the 3D-printed hydrogels were dependent on fish-dECM contents, where higher fish-dECM in the hydrogel increased swelling ratios and mass erosion rates. The higher content of fish-dECM considerably enhanced the viability of the incorporated cells in the matrix for 7 days. Artificial human skin was constructed by seeding human dermal fibroblasts and keratinocytes in the 3D-printed hydrogels, and a formation of a bilayered skin was visualized with tissue staining. Thus, we envision that 3D-printed hydrogels containing fish-dECM can be an alternative bioink composed of a non-mammal-derived matrix.

| S-EPMC10308565 | biostudies-literature

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