Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Effect of depletion of RAD51 on gene expression in Huh7 cells

ABSTRACT: To investigate the role of RAD51 in HCC, we established Huh7 cell lines in which RAD51 has been knocked down(KD) by sgRNA,we performed RNA-seq for RAD51-KD Huh7 cells and parental Huh7 cells(control group,sgcon).

ORGANISM(S): Homo sapiens

PROVIDER: GSE216728 | GEO | 2023/05/17

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

KCa3.1 depletion effect on hepatocellular carcinoma cell line Huh7

Project description:Analysis of Huh7 hepatocellular carcinoma (HCC) cells depleted for potassium channel KCa3.1, the intermediate conductance calcium-activated potassium channel. Result provide insight into the role of KCa3.1 in the carcinogenesis of HCC. We used microarrays to detail the gene expression differences between KCa3.1 knockdown and negative control in Huh7 HCC cells.

2018-06-23 | GSE116183 | GEO

Gene expression in the Bmi1 knockout Huh7 and Hep3B cells

Project description:Bmi1 plays a pivotal role in hepatic carcinoma (HCC), but its targets in HCC is unknown. To screen the potential targets, we transfected HCC cell line Huh7 and Hep3B with Bmi1 shRNA lenti-virus. After confirming the Bmi1 was knocked down using western blotting, we extracted total RNA and then run the microarray detection. Gene expression profiles in Bmi1 KO cells were compared with those in Bmi1 WT cells to screen potential targets of Bmi1.

2018-03-29 | GSE97169 | GEO

Effect of depletion of METTL16 on gene expression of Huh7 liver cancer cells [RNA-seq]

Project description:To investigate the mechanism of METTL16 in the regulation of tranlation and HCC development, we established Huh7 cell lines following METTL16 knockout (KO) by CRISPER-Cas9 system. We then performed gene expression profiling analysis using data obtained from RNA-seq of Huh7 cells.

2024-01-30 | GSE224008 | GEO

RNA-seq analysis of the gene expression profile of Huh7 cells upon incubation with different TGF-β-treated hepatic stellate cells (HSCs)-derived ectosomes

Project description:Purpose: The goals of this study are to compare the gene expression profile of Huh7 cells treated with different ectosomes or not and to evaluate the function of ectosomal Hexokianse 1 (HK1) derived from HSCs. Methods: Firstly, we treated different LX-2 HSCs with TGF-β (2 ng/mL) for 36 hours to activate HSCs, then ectosomes from LX-2 or HK1-KD LX-2 culture medium were isolated respectively by differential centrifugation processes. Secendly, Huh7 cells were preincubated with different ectosomes or not for 12 hours and then fresh medium for another 12 hours. Lastly, different groups of Huh7 cells were prepared for RNA-seq. Results: By Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis of the differentially expressed genes, a total of 124 pathways were found to be enriched in the ectosome-incubated Huh7 cells compared with the control Huh7 cells, while a total of 36 pathways were enriched in the ectosome-incubated Huh7 cells compared with the HK1-KD ectosome-incubated Huh7 cells.Gene set enrichment analysis (GSEA) also revealed enrichment of the N-glycosylation pathway in the ectosome-incubated Huh7 cells compared with the control Huh7 cells. Conversely, this pathway was negatively regulated in Huh7 cells incubated with HK1-KD HSC-derived ectosomes Conclusion: the transferal of HSC-derived ectosomal HK1 into HCC cells regulates the N-glycosylation level of Huh7.

2024-04-28 | GSE173632 | GEO

Gene expression profiles in CD44- and CD44+ hepatocellular carcinoma HuH7 cells

Project description:To investigate the significance of CD44+ hepatocellular carcinoma (HCC) HuH7 cells, gene expression profiles of CD44-positive HuH7, CD44-negative HuH7, and human nomal hepatocyes were analyzed. Results provide the insight into the significance of CD44-positive HCC cells as the liver CSCs.

2016-09-01 | GSE84223 | GEO

RNA-seq data for ABL1 knockdown in Huh7 cells

Project description:RNA-seq data for ABL1 knockdown (KD) in Huh7 cells

2021-06-30 | GSE133294 | GEO

RNA-seq data for EphA2 knockdown in Huh7 cells

Project description:RNA-seq data for EphA2 knockdown (KD) in Huh7 cells

2021-02-25 | GSE141880 | GEO

microRNA expression profiles in CD44- and CD44+ hepatocellular carcinoma HuH7 cells

Project description:To investigate the significance of CD44+ hepatocellular carcinoma (HCC) HuH7 cells, microRNA (miRNA) expression profiles of CD44-positive HuH7, CD44-negative HuH7, and human nomal hepatocyes were analyzed. Results provide the insight into the significance of CD44-positive HCC cells as the liver CSCs.

2016-09-01 | GSE84224 | GEO

Expression data comparing Huh7 control and CD47 knockdown cells

Project description:To identify candidate genes regulated by CD47 Affymetrix Human Genome U133 Plus GeneChip 2.0 HCC cell line Huh7 cells with or without CD47 repressed

2014-06-01 | E-GEOD-47563 | biostudies-arrayexpress

RNA-seq data for parental and lenvatinib-resistant HCC cells

Project description:RNA-seq data for parental and lenvatinib-resistant HCC (Hep3B, Huh7) cells

2022-03-23 | GSE186191 | GEO