Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Multiple genes of Candida albicans influencing echinocandin susceptibility in caspofungin-adapted mutants

ABSTRACT: Expression profiles of five caspofungin adapted candida albicans strains were analysed from their parental strains

ORGANISM(S): Candida albicans

PROVIDER: GSE217094 | GEO | 2022/11/05

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

Whole genome sequencing of caspofungin adaptors in Candida albicans

Project description:Approximately 200 cells of Candida albicans lab strain SC5314 were spread on YPD plates supplemented with 2 fold increase from 50 ng/ml to 12800ng/ml caspofungin. All adaptors appeared were collected. Randomly 36 tolerant adaptors were sequenced.

2023-09-15 | E-MTAB-12336 | biostudies-arrayexpress

Transcriptome profile of tra1 mutant in C. albicans in response to the antifungal caspofungin

Project description:The emergence of drug-resistant fungal strains is a threat to human health. Identifying targets that increase the susceptibility of fungal pathogens to current therapies will significantly improve outcomes. Tra1 is an essential component of the SAGA and NuA4 transcriptional co-activator complexes and is linked to multiple cellular processes associated with the yeast response to antifungal drugs. This submission contains RNA sequencing data from Candida albicans strain expressing a Tra1 mutant with three arginine to glutamine mutations in the PI3K domain (tra1Q3; Berg et al., 2018) treated with or without the antifungal caspofungin. Our goal was to identify genes regulated by Tra1 in C. albicans and identify genes with differential responses in the mutant tra1 strain upon capsofungin relative to the wild-type strain. We identified 68 genes that were differentially expressed when the tra1Q3 strain was treated with caspofungin, as compared to gene expression changes induced by either tra1Q3 or caspofungin alone. Included in this set were genes involved in cell wall maintenance, adhesion and filamentous growth. As a critical component of both SAGA and NuA4, Tra1 is uniquely positioned to regulate the yeast antifungal response and emerges, through targeting its PI3K domain, as a promising new therapeutic target for fungal infections.

2021-03-17 | GSE168988 | GEO

Whole genome sequencing of caspofungin adaptors derived from homozygous deletion strains of PKC and calcineurin pathways in Candida albicasn

Project description:In Candida albicans, PKC pathway genes MKK2 and MKC1, as well as calcineurin pathway genes CMP1 and CNB1 were deleted. The deletion strains were used to obtain caspofungin adaptors which gained tolerance to caspofungin. These adaptors as well as the wild type strain SC5314 were sequenced.

2023-02-13 | E-MTAB-12475 | biostudies-arrayexpress

Gcn5 Controls Virulence of the Human Fungal Pathogen Candida albicans through Multiple Regulatory Networks

Project description:Transcriptomic analysis of wild type and GCN5-deficient Candida albicans cells prior to and after treatment with Caspofungin 15 min and 45 min was done to determine the transcriptional changes in these strains

2019-07-26 | GSE123412 | GEO

RNA-Seq analysis of Candida glabrata wild-type and Cgset4Δ mutant in the presence and absence of the cell wall targeting echinocandin antifungal drug, caspofungin.

Project description:To determine the effect of caspofungin (CSP) treatment and/or loss of the SET domain-containing CgSet4 protein on the transcriptional response of log-phase C. glabrata cells. RNA-Seq analysis was conducted on CAA medium-grown log-phase Candida glabrata wild-type (wt) and CgSET4-deleted (Cgset4D) cells in the presence and absence of caspofungin.

2022-09-12 | GSE202654 | GEO

Whole genome sequencing of fluconazole tolerant adaptors derived from exposure to echinocandins in Candida albicans

Project description:Candida albicans lab strain SC5314 was spread on YPD plates supplemented with echinocandins (caspofungin, anidulafungin, micafungin, rezafungin). The adaptors were tested for tolrance to fluconazole. Randomly 43 tolerant adaptors, as well as the parent SC5314, were sequenced.

2023-07-11 | E-MTAB-12449 | biostudies-arrayexpress

Response of Candida albicans to taurocholate

Project description:Presence of taurocholate (TC), a constituent of bile, confers resistance of Candida albicans to antifungals like Caspofungin and Amphotericin B. Transcriptional profiles in presence and absence of TC were compared to determine whether an upregulation of known resistance genes is responsible for this effect. No upregulation was found, and the resistance seemed to be rather based on a direct interaction of TC with the antifungals.

2017-02-15 | E-MTAB-5277 | biostudies-arrayexpress

Caspofungin-induced Responses in the Planktonic Cells and Biofilms of Candida albicans

Project description:This project investigated the signature profiles of C. albicans planktonic cells and biofilms in response to caspofungin.

2021-01-08 | PXD023480 | iProX

Cell wall integrity is linked to mitochondria and phospholipid homeostasis in Candida albicans through the activity of the post-transcriptional regulator Ccr4-Pop2

Project description:The cell wall is essential for viability of fungi and is an effective drug target in pathogens such as Candida albicans. The contribution of posttranscriptional gene regulators to cell wall integrity in C. albicans is unknown. We show that the C. albicans Ccr4-Pop2 mRNA deadenylase, a regulator of mRNA stability and translation, is required for cell wall integrity. The ccr4/pop2 mutants display reduced wall β-glucans and sensitivity to the echinocandin caspofungin. Moreover, the deadenylase mutants are compromised for filamentation and virulence. We demonstrate that defective cell walls in the ccr4/pop2 mutants are linked to dysfunctional mitochondria and phospholipid imbalance. To further understand mitochondrial function in cell wall integrity, we screened a Saccharomyces cerevisiae collection of mitochondrial mutants. We identify several mitochondrial proteins required for caspofungin tolerance and find a connection between mitochondrial phospholipid homeostasis and caspofungin sensitivity. We focus on the mitochondrial outer membrane SAM complex subunit Sam37, demonstrating it is required for both trafficking of phospholipids between the ER and mitochondria and cell wall integrity. Moreover, in C. albicans also Sam37 is essential for caspofungin tolerance. Our study provides the basis for an integrative view of mitochondrial function in fungal cell wall biogenesis and resistance to echinocandin antifungal drugs.

2010-12-10 | GSE25891 | GEO

Whole genome sequencing of caspofungin adaptors in Candida parapsilosis

Project description:Candida parapsilosis wild type strain #12108 was exposed to 400ng/ml caspofungin. Randomly 30 adaptors were chosen. These adaptors as well as the parent were sequenced.

2022-11-05 | E-MTAB-12335 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data