Transcriptomic profile of WT and Mthfd2 KO alloreactive CD4+ T cells [RNA-seq]

ABSTRACT: To investigate the effect of Mthfd2 KO in alloreactive CD4+ T cells. We performed in vitro mixed lymphocyte to induce alloreactive T cells. CD4+ T cells were respectively purified from the spleens of 8-week-old TEaWT and TEaMthfd2 KO mice by magnetic-activated cell sorting (MACS) positive selection. APCs were sorted from the spleens of 8-week-old F1 (BALB/c x C57BL/6) mice. CD4+ T cells were cocultured with APCs three days and then performed RNA-sequencing.

ORGANISM(S): Mus musculus

PROVIDER: GSE217165 | GEO | 2025/09/01

REPOSITORIES: GEO