Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

RNA binding of DDX20 in the testis of postnatal 7-day-old C57BL/6 mice.

ABSTRACT: RNA immunoprecipitation assay followed by Next-Generation Sequencing (RIPseq) to identify RNAs bound by DDX20 in testis of postnatal 7-day-old C57BL/6 mice.

ORGANISM(S): Mus musculus

PROVIDER: GSE217687 | GEO | 2023/10/01

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

Identification of the embryonic germ cell Meioc-/- transcriptome, MEIOC targets in postnatal testis, and YTHDC2 targets in postnatal testis in mouse

Project description:Identification of the embryonic germ cell Meioc-/- transcriptome, MEIOC targets in postnatal testis, and YTHDC2 targets in postnatal testis in mouse

2017-03-22 | GSE90702 | GEO

DDX20 knockdown effect on hepatocellular carcinoma cells

Project description:Analysis of DDX20 knockdown - hepatocellular carcinoma cells. The expression levels of genes driven by NF-kB and related with carcinogenesis, were significantly enhanced in DDX20-knockdown cells. One condition experiment, HepG2 vs. HepG2-DDX20 knockdown cells

2012-07-14 | E-GEOD-28088 | biostudies-arrayexpress

Proteome of mouse testis at postnatal days 30

Project description:Proteome of mouse testis treated by arsenic from postnatal days 10 to 30.

2021-05-15 | PXD026031 | iProX

Phosphoproteome of mouse testis at postnatal days 30

Project description:Phosphoproteome of mouse testis treated by arsenic from postnatal days 10 to 30.

2021-05-15 | PXD026032 | iProX

DDX20 is required for cell cycle reentry of prospermatogonia and establishment of spermatogonial stem cell pool during testicular development in mice

Project description:DDX20 is required to promote spermatogonial stem cell pool formation by regulating mRNA translation during postnatal prospermatogonia differentiation in mice.

2024-02-01 | GSE244755 | GEO

Nanos2-associated mRNAs in the postnatal testis

Project description:To identify Nanos2-associated mRNAs, we performed microarray analysis of mRNAs coprecipitated with FLAG-tagged NANOS2 with postnatal testis from Tg-Nanos2 enhancer-3xFLAG-Nanos2-3'UTR at P7.

2015-09-25 | GSE61807 | GEO

Epigenome analysis of normal and DDX20 knockdown HepG2 cells

Project description:MicroRNAs (miRNAs) are small RNAs that regulate the expression of specific target genes. While deregulated miRNA expression levels have been detected in many tumors, whether miRNA functional impairment is also involved in carcinogenesis remains unknown. We investigated whether deregulation of miRNA machinery components and subsequent functional impairment of miRNAs are involved in hepatocarcinogenesis. Among miRNA-containing ribonucleoprotein complex components, reduced expression of DDX20 was frequently observed in human hepatocellular carcinomas, in which enhanced NF-kB activity is closely linked to carcinogenesis. Because DDX20 normally suppresses NF-kB activity by preferentially regulating the function of the NF-kB suppressing miRNA-140, we hypothesized that impairment of miRNA-140 function may be involved in hepatocarcinogenesis. Dnmt1 was identified as a direct target of miRNA-140, and increased Dnmt1 expression in DDX20-deficient cells hypermethylated the promoters of metallothionein genes, resulting in decreased metallothionein expression leading to enhanced NF-kB activity and hepatocarcinogenesis. MiRNA-140 knockout mice were prone to hepatocarcinogenesis and showed phenomena similar to those of DDX20 deficiency, suggesting that miRNA-140 plays a central role in DDX20 deficiency–related pathogenesis. Conclusion: These results indicate that miRNA-140 acts as a liver tumor suppressor, and that impairment of miRNA-140 function due to a deficiency of DDX20, a miRNA machinery component, could lead to hepatocarcinogenesis. Genome wide DNA methylation profiling of control and DDX20-knockdown HepG2 cells. Bisulphite converted DNA from the 2 samples were hybridised to the Illumina HumanMethylation450 BeadChip.

2012-04-26 | E-GEOD-37633 | biostudies-arrayexpress

DDX20 knockdown effect on hepatocellular carcinoma cells

2012-07-15 | GSE28088 | GEO

Epigenome analysis of normal and DDX20 knockdown HepG2 cells

2012-04-27 | GSE37633 | GEO

RNA-seq reveals an abnormal transcriptome as a result of DDX20 loss in spermatogonia.

Project description:To investigate the role of DDX20 in spermatogenesis, we generated Ddx20 flox/flox Ddx4-Cre mice to make a germ cell-specific Ddx20 knockout, and isolated spermatogonia from four-day-old mouse testes by THY1 magnetic beads.We then performed gene expression profiling analysis using data obtained from RNA-seq of THY1 + spermatogonia.

2023-10-01 | GSE217685 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data