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Rege-1 promotes C. elegans survival by modulating the metabolic pathways

ABSTRACT: To understand how excess amount of ets-4 contributed to poor survival of rege-1(ht070) fed with PA14, we performed mRNA-seq on the adult worms after 48 hours exposure to either OP50 or PA14 in all four strains.

ORGANISM(S): Caenorhabditis elegans

PROVIDER: GSE218235 | GEO | 2023/07/21

REPOSITORIES: GEO

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C. elegans gene expression in response to acute Pseudomonas aeruginosa infection (Part 1) and recovery (Part 2) by Neomycin/Streptomycin treatment

Project description:(Part 1) Gene expression profiles of C. elegans in response to P. aeruginosa. Synchronized larval stage 1 (L1) worms were raised on E. coli OP50 for 72 hours. These synchronized young adult (YA) animals were subsequently exposed to P. aeruginosa PA14 for 4 hours. 25dC. (Part 2) Gene expression profiles of C. elegans in response to Neomycin/Streptomycin-mediated recovery after a 4 hour exposure to P. aeruginosa. Synchronized larval stage 1 (L1) worms were raised on E. coli OP50 for 72 hours. These synchronized young adult (YA) animals were subsequently exposed to P. aeruginosa PA14 for 4 hours and then treated with Neomycin and shifted to E. coli OP50 plus Streptomycin plates for 6, 12, or 24 hours to resolve the infection. As a control for Neomycin/Streptomycin exposure, synchronized larval stage 1 (L1) worms were raised on E. coli OP50 for 72 hours and either shifted to fresh E. coli OP50 plates for 6 hours or treated with Neomycin and shifted to E. coli OP50 plus Streptomycin plates for 6 hours. 25dC.

2016-08-11 | GSE81592 | GEO

Quantitative Analysis of Worms after P. aeruginosa Infection

Project description:To clarify the mechanisms of innate immune responses mediated by epithelial barrier disruption. Reveal the host’s broad transcriptional response to P. aeruginosa infection during disruption of the intestinal barrier. We used RNA-Seq analysis to study the transcriptomic profiles of wt worms were exposed to either P. aeruginosa PA14 or E. coli OP50 for 24 hours, and the transcriptomic profiles of egl-44(MT2247) mutants fed E. coli OP50 for 24 hours. Differential expression analysis was performed by comparing wt worms exposed to PA14 versus wt worms exposed to E. coli OP50, and egl-44(MT2247) mutants fed E. coli OP50 versus WT worms fed E. coli OP50.

2018-10-11 | GSE121091 | GEO

Response of C. elegans immune pathway mutants to P. aeruginosa infection

Project description:Synchronized C. elegans cultures of three geontypes -- wildype N2, daf-2(e1370) and sma-6(wk7) -- were prepared using standard techniques (http://cmgm.stanford.edu/~kimlab/index_methods.html). Live young adult worms were split between NG plates pre-seeded with the non-pathogenic E. coli strain OP50 or the Pseudomonas aeruginosa clinical isolate PA14 and incubated at 25C for 4 or 24 hours before harvesting. This experiment was repeated at least three times on independent occasions. cDNA probes were prepared from experimental samples and from reference mRNA extracted from mixed stage wild type worms grown at 25C, and were labeled with Cy3 or Cy5, as indicated. A reference experiement design type is where all samples are compared to a common reference. Elapsed Time: Time Infection: Exposure to the non-pathogenic E. coli strain OP50 or to the pathogenic Pseudomonas aeruginosa strain PA14 Strain Name: C. elegans wildtype strain N2 or the immune pathway mutants daf-2(e1370) or sma-6(wk7) Keywords: reference_design Computed

2008-11-05 | E-GEOD-13473 | biostudies-arrayexpress

Response of C. elegans immune pathway mutants to P. aeruginosa infection

2008-11-06 | GSE13473 | GEO

RNA sequencing to identify UNC-25-regulated genes in C. elegans defense against pathogen P. aeruginosa infection

Project description:Purpose: To gain molecular insights on how UNC-25 regulates C. elegans innate immunity, we used RNA sequencing to profile gene expression in unc-25(e156) animals relative to wild-type animals with or without P. aeruginosa (PA14) infection. Methods: Synchronized L1 worms were placed onto NGM plates seeded with E. coli OP50 and grown at 20 °C until the L4 stage. Worms were washed off the plates, collected with M9 solution and subsequently transferred to modified NGM plates containing E. coli OP50 or P. aeruginosa PA14 for 24 h at 25 °C. Then, the animals were washed off the plates with M9 solution and frozen in TRIzol (Transgen, ER501-01, China) with liquid nitrogen. Total RNA was isolated using a TransZol Up Plus RNA kit (Transgen, ER501-01, China). Results: RNA seq analyses shows enriched and signficant changed immune genes, neuropeptides, transcription factors and pathways that are dependent of GABA signaling. Conclusion: Our studies uncover that GABA signaling enhance the resistence to pathogen induced killing.

2023-06-26 | GSE195835 | GEO

Ferritin regulation upon ETS-4/SPDEF depletion promotes cold survival in C. elegans

Project description:In this study, we subjected wild type worms and worms deficient in ETS-4, ETS-4 and DAF16, ETS-4 and PQM-1, to cold treatment and analyzed their transcriptional profiles by RNAseq.

2019-05-29 | GSE131870 | GEO

Transcriptional profiling of C. elegans on pathogenic Pseudomonas aeruginosa

Project description:RNA-seq of Wild Type (N2), pmk-1 or atf-7 mutant animals exposed to either non-pathogenic E. coli OP50 or pathogenic P. aeruginosa PA14

2019-02-01 | GSE119292 | GEO

C. elegans gene expression in response to the pathogenic P. aeruginosa strain PA14.

Project description:Analysis of differential gene expression in C. elegans adults exposed to three different bacteria: E. coli strain OP50, wild-type P. aeruginosa PA14 and gacA mutant PA14. Samples were analyzed at 4 hours and 8 hours after exposure to the different bacteria. These studies identified C. elegans genes induced by pathogen infection. Keywords: Time course, response to pathogen infection

2007-02-08 | GSE5793 | GEO

Binding profile of the bZIP transcription factor, ATF-7, in C. elegans on pathogenic Pseudomonas aeruginosa

Project description:ChIP-seq of ATF-7::GFP from Wild Type or pmk-1 mutant animals exposed to either non-pathogenic E. coli OP50 or pathogenic P. aeruginosa PA14

2019-02-01 | GSE119293 | GEO

Protein EV contents from C. elegans

Project description:The hermaphrodite strain N2 (Bristol), fed with Escherichia coli OP50, was maintained and synchronized. Young adults were thoroughly washed before incubations for 5 h or 24 h in M9 buffer. Between 35,500 and 158,000 worms were used for each experiment. After incuabtion, supernatants were processed by differential centrigugation/ultracentrifugation to pellet extracellular vesicles and characterization of the protein contents by mass spectrometry.

2020-05-04 | PXD017352 | Pride

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