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Microarray analysis of bovine macrophages cell line (BoMac) after infection with Bovine Immunodeficiency Virus

ABSTRACT: Bovine Immunodeficiency Virus (BIV) is a member of Retroviruses family which is natural pathogen of cattle, prevalent worldwide. The significance of BIV infection in cattle has not been clearly established and it is still unknown whether BIV induces a specific syndrome or whether it renders animals more susceptible to other infections. To gain insight into host response to BIV infection the pattern of gene expression in bovine macrophage cells (BoMac) was analyzed using BLO Plus microarrays from CAFG, Michigan State University ( GEO GPL9176).

ORGANISM(S): Bos taurus

PROVIDER: GSE218260 | GEO | 2022/11/21

REPOSITORIES: GEO

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Project description:Background: African animal trypanosomiasis (AAT) caused by tsetse fly-transmitted protozoa of the genus Trypanosoma is a major constraint on livestock and agricultural production in Africa and is among the top ten global cattle diseases impacting on the poor. Here we show that a functional genomics approach can be used to identify temporal changes in host peripheral blood mononuclear cell (PBMC) gene expression due to disease progression. We also show that major gene expression differences exist between cattle from trypanotolerant and trypanosusceptible breeds. Using bovine long oligonucleotide microarrays and real time quantitative reverse transcription PCR (qRT-PCR) validation we analysed PBMC gene expression in naïve trypanotolerant and trypanosusceptible cattle experimentally challenged with Trypanosoma congolense across a 34-day infection time course. Results: Trypanotolerant N’Dama cattle displayed a rapid and distinct transcriptional response to infection, with a ten-fold higher number of genes differentially expressed at day 14 post infection compared to trypanosusceptible Boran cattle. These analyses identified coordinated temporal gene expression changes for both breeds in responses to trypanosome infection. In addition, a panel of genes were identified that showed pronounced differences in gene expression between the two breeds, which may underlie the phenomena of trypanotolerance and trypanosusceptibility. Gene ontology (GO) analysis demonstrate that the products of these genes may contribute to increased mitochondrial mRNA translational efficiency, a more pronounced B cell response, an elevated activation status and a heightened response to stress in trypanotolerant cattle. Conclusions: This study has revealed an extensive and diverse range of cellular processes that are altered temporally in response to trypanosome infection in African cattle. Results indicate that the trypanotolerant N’Dama cattle respond more rapidly and with a greater magnitude to infection compared to the trypanosusceptible Boran cattle. Specifically, a subset of the genes analyzed by qRT-PCR, which display significant breed differences, could collectively contribute to the trypanotolerance trait in N’Dama.

Dataset Information

Microarray analysis of bovine macrophages cell line (BoMac) after infection with Bovine Immunodeficiency Virus

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