Single-Cell RNA Sequencing Analysis of Human Embryonic Stem Cell-Derived Neural Organoids

ABSTRACT: Human pluripotent stem cell-derived 3D neural organoids have been shown to recapitulate the major features and cytoarchitecture of early human brain development. We used three commercially available kits: STEMdiff™ Cerebral Organoid Kit, STEMdiff™ Dorsal Forebrain Organoid Differentiation Kit, and STEMdiff™ Ventral Forebrain Organoid Differentiation Kit. These neural organoids contain unique and diverse cell types which model the early brain and are patterned with developmental cues. Here, we sought to investigate the cellular diversity of these organoids using single-cell RNA sequencing.

ORGANISM(S): Homo sapiens

PROVIDER: GSE218457 | GEO | 2023/02/23

REPOSITORIES: GEO