Transcriptomics

Dataset Information

0

Elevated pre-mRNA 3' end processing activity in cancer cells renders vulnerability to inhibition of cleavage and polyadenylation [QuantSeq]


ABSTRACT: Cleavage and polyadenylation (CPA) defines the 3’ end of almost all eukaryotic mRNAs. CPA inhibition, or CPAi, leads to transcriptional readthrough. Here, we show that the CPSF-73 inhibitor JTE-607 globally perturbs gene expression, especially for those with a high GC content and located in high gene density regions. Based on regulated alternative polyadenylation (APA) events, we found that more frequently used CPA sites are inhibited by JTE-607 to a greater extent. Consistently, cells with elevated CPA activities, as indicated by preferential usage of proximal APA sites, display greater transcriptional readthrough and gene expression disturbance upon JTE-607 treatment. Remarkably, overexpression of the core CPA factor FIP1 enhances global CPA activity in the cell and leads to greater JTE-607 sensitivity. Taken together, our data indicate that CPAi selectively impacts genes based on their genomic features and the CPA activity of a cell is a key determinant of sensitivity to CPAi.

ORGANISM(S): Homo sapiens

PROVIDER: GSE218555 | GEO | 2023/06/07

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1

Similar Datasets

2023-06-07 | GSE231527 | GEO
2023-06-07 | GSE218545 | GEO
2021-01-17 | GSE164958 | GEO
2021-09-09 | PXD018090 | Pride
2015-04-01 | E-GEOD-62001 | biostudies-arrayexpress
2024-01-06 | GSE252667 | GEO
2015-04-01 | GSE62001 | GEO
2021-01-28 | GSE151722 | GEO
2021-01-28 | GSE151721 | GEO
2021-01-28 | GSE151720 | GEO