Similar Datasets
Project description:These are 3 TMT10 samples. The indicated "standard" sub-samples all refer to the same biological sample (obtained as a mixture of all other samples), split and tagged as indicated, to play as a reference for normalization.
TMT10 sample1 contains the following sub-samples:
-TAG 126: Standard.
-TAG 127N: Day 3 conditioned medium of replication-incompetent MEFs cultured on VTN coating in RSeT medium.
-TAG 127C: Day 3 conditioned medium of naive human pluripotent stem cells cultured on a MEF feeder-layer in RSeT medium.
-TAG 128N: Day 3 conditioned medium of naive human pluripotent stem cells cultured on a VTN coating in RSeT medium.
-TAG 128C: Not relevant.
-TAG 129N: Not relevant.
-TAG 129C: Not relevant.
-TAG 130N: Not relevant.
-TAG 130C: RSet medium.
-TAG 131: Standard.
TMT10 sample2 contains the following sub-samples:
-TAG 126: Standard
-TAG 127N: Day 3 conditioned medium of replication-incompetent MEFs cultured on VTN coating in RSeT medium.
-TAG 127C: Day 3 conditioned medium of naive human pluripotent stem cells cultured on a MEF feeder-layer in RSeT medium.
-TAG 128N: Day 3 conditioned medium of naive human pluripotent stem cells cultured on a VTN coating in RSeT medium.
-TAG 128C: Not relevant.
-TAG 129N: Day 2 conditioned medium of naive human pluripotent stem cells cultured on a MEF feeder-layer in RSeT medium.
-TAG 129C: Day 2 conditioned medium of naive human pluripotent stem cells cultured on a VTN coating in RSeT medium.
-TAG 130N: Not relevant.
-TAG 130C: RSet medium.
-TAG 131: Standard.
TMT10 sample3 contains the following sub-samples:
-TAG 126: Standard.
-TAG 127N: Day 3 conditioned medium of replication-incompetent MEFs cultured on VTN coating in RSeT medium.
-TAG 127C: Day 3 conditioned medium of naive human pluripotent stem cells cultured on a MEF feeder-layer in RSeT medium.
-TAG 128N: Day 2 conditioned medium of replication-incompetent MEFs cultured on VTN coating in RSeT medium.
-TAG 128C: Day 2 conditioned medium of replication-incompetent MEFs cultured on VTN coating in RSeT medium.
-TAG 129N: Day 2 conditioned medium of naive human pluripotent stem cells cultured on a MEF feeder-layer in RSeT medium.
-TAG 129C: Day 2 conditioned medium of naive human pluripotent stem cells cultured on a VTN coating in RSeT medium.
-TAG 130N: Not relevant.
-TAG 130C: RSet medium.
-TAG 131: Standard.
Detailed culture and processing methods are described in Cesare et al, under submission.
| MSV000086068 | MassIVE
Project description:Expression profiling by bulk RNA sequencing
| PRJNA906188 | ENA
Project description:Bulk RNAseq of 3 replicates of primed H9 +/- EZH2i (E8Flex) and 3 replicates of naive H9 +/- EZH2i (PXGL) to assess the effect of EZH2i in human pluripotent stem cells
2022-03-23 | GSE176172 | GEO
Project description:We report raw bulk RNA sequencing data of rice root sections from Meristem, Elongation and Maturation zones. For each section, we include 6 biological replicates. We also include the whole-root samples that include all developmental zones. The genes with enriched expression in certain developmental zones were used as developmental stage markers for our following single-cell RNA sequencing annotation.
2024-07-01 | E-MTAB-13889 | biostudies-arrayexpress
Project description:This is a TMT6 sample containing the following sub-samples:
TAG 126: standard, used for normalization purposes, It is a mixture of different biological samples.
TAG 127: Lysate of replication-incompetent MEFs cultured on VTN coating in RSeT medium.
TAG 128: Lysate of naive human pluripotent stem cells cultured on a MEF feeder-layer in RSeT medium (mixed human and mouse cell population).
TAG 129: Lysate of naive human pluripotent stem cells cultured on VTN coating in RSeT medium.
TAG 130: Not relevant.
TAG 131: standard, used for normalization purposes, It is a mixture of different biological samples. Same as sub-sample with tag 126.
Detailed culture and processing methods are described in Cesare et al, under submission.
| MSV000086069 | MassIVE
Project description:We report the application of bulk RNA sequencing technology for high-throughput profiling of SARS-CoV-2 infection in the human nasopharynx
2024-07-27 | GSE239595 | GEO
Project description:We report the application of bulk RNA sequencing technology for high-throughput profiling of SARS-CoV-2 infection in the lung of Syrian golden hamsters (Mesocricetus auratus).
2022-05-26 | GSE204696 | GEO
Project description:Induced pluripotent stem cells (iPSCs) are usually clonally derived. The selection of fully reprogrammed cells generally involves picking of individual colonies with morphology similar to embryonic stem cells (ESCs). However, successfully reprogrammed cells are highly proliferative and escape from cellular senescence - it is therefore conceivable that they outgrow non-pluripotent and partially reprogrammed cells during culture expansion without the need of clonal selection. In this study, we have reprogrammed human dermal fibroblasts (HDFs) with episomal plasmid vectors. Colony frequency and size was higher when using murine embryonic fibroblasts (MEFs) as stromal support instead of HDFs or human mesenchymal stromal cells (MSCs). We have then compared iPSCs which were either clonally derived by manual selection of a single colony, or derived from bulk-cultures of all initial colonies. After few passages their morphology, expression of pluripotency markers, and gene expression profiles did not reveal any significant differences. Furthermore, clonally-derived and bulk-cultured iPSCs had indistinguishable in vitro differentiation potential towards the three germ layers. Therefore, manual selection of individual colonies does not appear to be necessary for the generation of iPSCs M-bM-^@M-^S this is of relevance for standardization and automation of cell culture procedures Gene expression profiles of induced fibroblasts (M-bM-^@M-^\iFM-bM-^@M-^]) from three different donors which were either clonally derived (M-bM-^@M-^\CM-bM-^@M-^]) or bulk-cultured (M-bM-^@M-^\BM-bM-^@M-^]) were compared
2013-06-03 | E-GEOD-42807 | biostudies-arrayexpress
Project description:Transcriptional Profiling of human pluripotent stem cells and and derived tissues
2025-01-23 | E-MTAB-3158 | ExpressionAtlas
Project description:RNA-Seq of GNRH1 neurons differentiated from human pluripotent stem cells
2025-01-23 | E-MTAB-7938 | ExpressionAtlas