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BET inhibition abrogates global transcription via repressing m6A writer complex

ABSTRACT: To characterize the role of m6A modification in mediating BRD4-dependent biological functions, we established METTL3 reconstituting cells and treated with JQ1 BRD4 directs gene transcription through diverse mechanisms. Here, we specifically focused on BETi-dependent transcriptome or chestrated by m6A-mediated regulation. To this end, we first need to identify bona fide m6A-dependent transcripts via rescue experiments in METTL3-knockdown cells by adding back wild-type METTL3 or METTL3-CD mutant (a catalytically inactive form of METTL3)

ORGANISM(S): Homo sapiens

PROVIDER: GSE219191 | GEO | 2023/12/31

REPOSITORIES: GEO

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