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Kinetics of gene expression after light exposure in hub1-3 mutant compared to wild-type

ABSTRACT: au10-03_hub1 - kinetics of gene expression after light exposure in hub1-3 mutant compared to wild-type - Role of histone UbH2B in the regulation of gene expression in response to light - RNA from 5-day-old wild-type and hub1-3plants in 3 conditions were used for hybridization: 5 days of darkness, 5 days of darkness + 1 hour of light and 5 days of darkness + 6 hours of light. Keywords: genotype comparaison,time course

ORGANISM(S): Arabidopsis thaliana

PROVIDER: GSE21922 | GEO | 2011/05/20

SECONDARY ACCESSION(S): PRJNA126805

REPOSITORIES: GEO

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kinetics of gene expression after light exposure in hub1-3 mutant compared to wild-type

Project description:au10-03_hub1 - kinetics of gene expression after light exposure in hub1-3 mutant compared to wild-type - Role of histone UbH2B in the regulation of gene expression in response to light - RNA from 5-day-old wild-type and hub1-3plants in 3 conditions were used for hybridization: 5 days of darkness, 5 days of darkness + 1 hour of light and 5 days of darkness + 6 hours of light. Keywords: genotype comparaison,time course 10 dye-swap - CATMA arrays

2011-05-20 | E-GEOD-21922 | biostudies-arrayexpress

della-regulation in darkness versus light-DELLAs contribute to skotomorphogenesis

Project description:rs09-09_della-dark - della-regulation in darkness versus light - Identification of DELLA-dependent downstream targets in darkness - Aim was to determine downstream target of DELLA proteins involved in skotomorphogenesis. Wt, ga1-3, ga1-3_rga_gai_rgl1_rgl2_rgl3 global seeds were sterilized, sown on MS agar plates then put for stratification for 3 days at 4°C. Plates were placed in growth cabinet at 22°C for 5 days in darkness or in continous light. Keywords: gene knock out

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Transcriptome profiles of different light-signaling mutants

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The conserved ubiquitin-like protein Hub1 plays a critical role in splicing in human cells

Project description:Different from canonical ubiquitin-like proteins, Hub1 does not form covalent conjugates with substrates but binds proteins non-covalently. In Saccharomyces cerevisiae, Hub1 associates with spliceosomes and mediates alternative splicing of SRC1, without affecting pre-mRNA splicing generally. Human Hub1 is highly similar to its yeast homolog, but its cellular function remains largely unexplored. Here, we show that human Hub1 binds to the spliceosomal protein Snu66 as in yeast, however, unlike its S. cerevisiae homolog, human Hub1 is essential for viability. Prolonged in vivo depletion of human Hub1 leads to various cellular defects, including splicing speckle abnormalities, partial nuclear retention of mRNAs, mitotic catastrophe and consequently cell death by apoptosis. Early consequences of Hub1 depletion are severe splicing defects, however, only for specific splice sites leading to exon skipping and intron retention. Thus, the ubiquitin-like protein Hub1 is not a canonical spliceosomal factor needed generally for splicing, but rather a modulator of spliceosome performance and facilitator of alternative splicing.

2014-05-30 | GSE56878 | GEO

Effect of light on gene expression in Schizophyllum commune

Project description:In order to study the effect of light on gene expression in Schizophyllum commune, genome wide gene expression was analysed in 4-day-old monokaryotic and dikaryotic wild type colonies, grown either in light or in darkness. 4 samples: - monokaryon, grown for 4 days in the light - monokaryon, grown for 4 days in the dark - dikaryon, grown for 4 days in the light - dikaryon, grown for 4 days in the dark RNA was obtained from 3 biological replicates and pooled

2013-08-12 | E-GEOD-23533 | biostudies-arrayexpress

Effect of light on gene expression in Schizophyllum commune

2013-08-12 | GSE23533 | GEO

Transcriptional response of Arabidopsis seedlings to light

Project description:We report the transcriptomic response of Arabidopsis thaliana seedlings grown in continuous white light, darkness or darkness and 1 hour of red light

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Expression profiles of dark or red-light treated WT and phytochrome mutants

Project description:The mRNA expression profiles of the wild type, phyB and phyAB mutants were analyzed with darkness or 1 hour red light treatment. Results provide insight into the role of red-light and phytochromes in gene expression.

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Light and BcLTF2-dependent gene expression profiles in the phytopathogenic fungus Botrytis cinerea

Project description:Full transcriptomes of the Botrytis cinerea wild-type strain B0510 and the null-mutant deltaBcLTF2, cultured in continuous darkness or submitted to a light stimulation, were compared to identify light- or/and BcLTF2-dependent genes.

2016-06-01 | GSE79679 | GEO

Light and BcLTF1-dependent gene expression profiles in the phytopathogenic fungus Botrytis cinerea

Project description:Full transcriptomes of the Botrytis cinerea wild-type strain B0510 and the null-mutant deltaBcLTF1, cultured in continuous darkness or submitted to a light stimulation, were compared to identify light- or/and BcLTF1-dependent genes.

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