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H3K27Ac ChIP-seq in wild-type and XAF1 knockout HT29 cells after vesicular stomatitis virus infection

ABSTRACT: To investigate the function of XAF1 in chromatin openning. Wild-type or XAF1 knockout HT29 cells were infected with VSV for 0 or 3 hours, ChIP-seq was did with H3K27Ac antibody. Chromatin immunoprecipitation DNA-sequencing (ChIP-seq) for histone modifications H3K27Ac in HT29 cells.

ORGANISM(S): Homo sapiens

PROVIDER: GSE220272 | GEO | 2023/01/01

REPOSITORIES: GEO

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ATAC-seq in wild-type and XAF1 knockout HT29 cells after vesicular stomatitis virus infection

Project description:To investigate the function of XAF1 in chromatin openning. Wild-type or XAF1 knockout HT29 cells were infected with VSV for 0 or 3 hours,ATAC-seq was performed. Examination of chromatin openness in Wild-type or XAF1 knockout HT29 cells.

2023-03-01 | GSE225587 | GEO

mRNA-seq of wild-type or XAF1 knockout HT29 cells after vesicular stomatitis virus infection

Project description:To investigate the function of XAF1 in immune response, we established XAF1 knockout cell lines in which XAF1 gene has been knockout by sgRNA. We then performed gene expression profiling analysis using data obtained from RNA-seq of WT or XAF1 knockout HT29 cells with or without VSV infection

2023-01-01 | GSE220273 | GEO

XAF1 knockout HT29 cells after vesicular stomatitis virus infection

Project description:This SuperSeries is composed of the SubSeries listed below.

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miRNA profiling of mouse primary peritoneal macrophages infected with vesicular stomatitis virus (VSV)

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Project description:Heterogeneous nuclear ribonucleoproteins (hnRNPs) are involved in many processes in RNA metabolism. In addition to their functions in the nucleus, hnRNPs can function in the replication of RNA viruses in the cytoplasm. In vesicular stomatitis virus (VSV)-infected cells, several hnRNPs relocalize from the nucleus to the cytoplasm. This raises the question of whether these hnRNPs are relocalized together with their host nuclear RNAs or whether they associate with new RNAs in the cytoplasm. hnRNP A1, hnRNP C1/C2, and hnRNP K were immunoprecipitated from mock- or VSV-infected cells, and RNAs were analyzed by high content RNA sequencing. Each hnRNP displayed a loss of interaction with cellular transcripts in favor of viral mRNAs. hnRNP A1 was preferentially associated with VSV phosphoprotein (P) mRNA; hnRNP C1/C2 was preferentially associated with VSV glycoprotein (G) mRNA, and hnRNP K bound viral transcripts in rank of abundance.

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Project description:Infected and Uninfected with VSV

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H3K27Ac ChIP-seq in wild-type and SAFA-/- cells

Project description:wild-type or SAFA-/- THP-1 cells were infected with VSV for 8 hours or 24 hours, ChIP-seq was did with H3K27Ac antibody.

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Expression data from C57BL/6J and C57BL6/J Sarm-deficient mice uninfected or infected with vesicular stomatitis virus (VSV)

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