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Drought Recovery in Plants Triggers a Cell-State Specific Immune Activations [bulk RNA-seq]

ABSTRACT: To elucidate the transcriptional landscape during drought recovery we sequenced a fine-scale time-course of drought recovering plants.

ORGANISM(S): Arabidopsis thaliana

PROVIDER: GSE220276 | GEO | 2025/07/23

REPOSITORIES: GEO

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Drought Recovery in Plants Triggers a Cell-State Specific Immune Activation [RNA-seq]

Project description:All organisms experience stress as an inevitable part of life, from single-celled microorganisms to complex multicellular beings. The ability to recover from stress is a fundamental trait that determines the overall resilience of an organism, yet stress recovery is understudied. To understand the stress recovery process, we studied the recovery from drought stress in Arabidopsis thaliana. We performed a fine-scale time series of bulk RNA sequencing starting 15 minutes after rehydration following moderate drought. We reveal that drought recovery is a rapid process involving the activation of thousands of recovery-specific genes. To capture these rapid recovery responses in different leaf cell types, we performed single-nucleus transcriptome analysis at the onset of post-drought recovery, identifying a cell type-specific transcriptional state developing independently across cell types. Furthermore, we reveal a recovery-induced activation of the immune system that occurs autonomously, and which enhances pathogen resistance in vivo in A. thaliana, wild tomato (Solanum pennellii) and domesticated tomato (Solanum lycopersicum cv. M82). Since rehydration promotes microbial proliferation and thereby increases the risk of infection, the activation of drought recovery-induced immunity may be crucial for plant survival in natural environments. These findings indicate that drought recovery coincides with a preventive defense response, unraveling the complex regulatory mechanisms that facilitate stress recovery in different plant cell types.

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Drought Recovery in Plants Triggers a Cell-State Specific Immune Activation [MERFISH]

Project description:Rain-fed plants are subjected to cycles of drought and re-watering. Thus, efficient recovery from drought may be among the key determinants in the success of these plants, yet the mechanisms by which plant recover from drought have been historically understudied. To parse out the specific molecular processes leading to stress recovery, we performed a fine-scale time course of bulk RNA sequencing starting just 15 minutes after rehydration. We revealed that transcriptional drought recovery is an active and rapid process involving activating thousands of recovery-specific genes. To map the immediate recovery responses in diverse leaf-cell-types, we performed a single-nucleus transcriptome analysis of the onset of recovery from long-term moderate drought. Here we show the formation of unique cell states initiated rapidly following plant rehydration. We reveal the activation of a microbial-autonomic induction of the immune system and demonstrate that the onset of recovery leads to increased resistance against pathogens in Arabidopsis (Arabidopsis thaliana), wild tomato (Solanum pennellii) and domesticated tomato (Solanum lycopersicum cv. M82). Our findings uncover that the preventive immune activation was preserved despite extensive selection during tomato domestication. Since rehydration increases microbial proliferation and, thus, the risk for infection, pre-activating immunity may be crucial for plants survival in natural environments. This work lays the foundation for unraveling the complexed processes actively facilitating stress recovery within plant leaves.

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Drought Recovery in Plants Triggers a Cell-State Specific Immune Activation [snRNA-Seq]

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Drought Recovery in Plants Triggers a Cell-State Specific Immune Activations [bulk RNA-seq]

Project description:Drought Recovery in Plants Triggers a Cell-State Specific Immune Activations [bulk RNA-seq]

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Medicago truncatula drought stress recovery

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Drought Recovery in Plants Triggers a Cell-State Specific Immune Activation

Project description:This SuperSeries is composed of the SubSeries listed below.

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