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Genome-wide Chromatin Maps Derived from Limited Numbers of Hematopoietic Progenitors

ABSTRACT: Whole genome mapping of protein-DNA interactions can be performed by coupling chromatin immunoprecipitation (ChIP) with high-throughput sequencing (ChIP-Seq). However, current methods require large amounts of starting materials which precludes their application to rare cell types. Here, we combine a high-sensitivity ChIP assay with a novel library preparation procedure to map histone modifications in as few as 10,000 cells. We apply the technique to acquire genome-wide chromatin maps for an enriched population of hematopoietic progenitors, and thereby gain insight into their developmental program.

ORGANISM(S): Mus musculus

PROVIDER: GSE22075 | GEO | 2010/07/01

SECONDARY ACCESSION(S): PRJNA128955

REPOSITORIES: GEO

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Genome-wide Chromatin Maps Derived from Limited Numbers of Hematopoietic Progenitors

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