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The role of altered lipid composition and distribution in liver fibrosis revealed by multimodal nonlinear optical microscopy

ABSTRACT: To reveal the involved gene regulatory pathways, we carried out RNA-seq transcriptomics analysis on the cells treated with or without PA, treated with PA+OA and PA+BMS.

ORGANISM(S): Homo sapiens

PROVIDER: GSE220856 | GEO | 2022/12/22

REPOSITORIES: GEO

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Phototoxic effects of nonlinear optical microscopy on cell cycle, oxidative states, and gene expression

Project description:Nonlinear optical imaging modalities, such as stimulated Raman scattering (SRS) microscopy, use pulsed-laser excitation with high peak intensity that can perturb the native state of cells. In this study, we used bulk RNA sequencing, quantitative measurement of cell proliferation, and fluorescent measurement of the generation of reactive oxygen species (ROS) to assess phototoxic effects of near-IR pulsed laser radiation, at different time scales, for laser excitation settings relevant to SRS imaging. We define a range of laser excitation settings for which there was no significant change in mouse Neuro2A cells after laser exposure. This study provides guidance for imaging parameters that minimize photo-induced perturbations in SRS microscopy to ensure accurate interpretation of experiments with time-lapse imaging or with paired measurements of imaging and sequencing on the same cells.

2022-03-06 | GSE197666 | GEO