Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Combined transcriptome and T cell receptor alpha/beta (TRA/TRB) single cell RNA sequencing of donor-reactive T cells in stable kidney transplant recipients before and 3-5 years post-transplant.

ABSTRACT: A combined single cell RNA sequencing approach determined both transcriptomic profiles and complete V(D)J variable regions of TR transcripts from individual alloreactive T cells of stable kidney transplant recipients (N=7) prior to and 3-5 years after transplantation

ORGANISM(S): Homo sapiens

PROVIDER: GSE220956 | GEO | 2023/10/23

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

Combined transcriptome and T cell receptor alpha/beta (TRA/TRB) single cell RNA sequencing of donor-reactive T cells in stable kidney transplant recipients before and 3-5 years post-transplant.

Project description:Combined transcriptome and T cell receptor alpha/beta (TRA/TRB) single cell RNA sequencing of donor-reactive T cells in stable kidney transplant recipients before and 3-5 years post-transplant.

| PRJNA912159 | ENA

Pre-Transplant T-Cell Clonal Analysis Identifies CD8+ Donor Reactive Clones That Contribute To Kidney Transplant Rejection Following Two Different Induction Modalities

Project description:Introduction: Responses to allogeneic human leukocyte antigen (HLA) molecules limit the survival of transplanted organs. The changes in T-cell alloreactivity that contribute to this process, however, are not fully understood. We defined a set of donor reactive T-cell clones (DRTC) with the goal to elucidate signatures of kidney allograft rejection. Methods: DRTC were identified pretransplant using an anti-donor mixed lymphocyte reaction assay: CFSE-diluting CD4+ and CD8+ DRTC were flow-sorted, and the TCR sequences were identified using Adaptive Immunosequencing. DRTC were then tracked in post-transplant biopsies, blood, and urine samples in a cohort of kidney transplant recipients. Results: In patients with an abnormal biopsy, the majority of CD8+ DRTC found within the allograft were detected in the circulating pre-transplant repertoire. Circulating CD8+ DRTC were more abundant pre- and post-transplant in patients that received non-lymphodepletional induction and developed an abnormal biopsy when compared to stable patients. Additionally, DRTC were detected as early as two weeks post-transplant in the urine of some patients, with some of these clones subsequently identified in follow-up kidney biopsy samples. Discussion: The findings of our study add to our understanding of T-cell alloreactivity following kidney transplantation and provide evidence for the role of pre-defined alloreactive T-cells in the development of allograft rejection.

2025-02-10 | GSE288648 | GEO