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Nodal coordinates the anterior-posterior patterning of germ layers and induces head formation in zebrafish explants.

ABSTRACT: Purpose: To investingate cell types in wild-type zebrafish explants. Methods: Wild-type explants (injected with phenol red) were harvested at 10hpf. Libraries were prepared using Chromium Controller and Chromium Single Cell 3’Library & Gel Bead Kit v3 (10x Genomics, PN-1000075) according to the manufacturer’s protocol for 10000 cells recovery. Results: A total of 9,968 single-cell transcriptomes were collected after stringent quality control measures. Conclusions: 6 Cell types were identified, wild-type explants mainly contains the anterior neural ectoderm and epidermis at 10 hpf.

ORGANISM(S): Danio rerio

PROVIDER: GSE221220 | GEO | 2022/12/22

REPOSITORIES: GEO

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