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Single cell RNA sequencing profiles of human iPSC-derived AT1-like cells in 3D cultures [SPC2B2_LDCI]

ABSTRACT: Comparison of differentiation of human iPSC derived alveolar epitheilal type 2 cells (iAT2s) into AT1-like cells (iAT1s) using a defined "LDCI" medium vs. lentiviral forced over-expression of activated nuclear YAP (YAP5SA)

ORGANISM(S): Homo sapiens

PROVIDER: GSE221343 | GEO | 2023/08/08

REPOSITORIES: GEO

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Nuclear YAP overexpression drives the human alveolar type 1 cell (AT1) cprogram in a cell autonomous manner in lentiviral transduced iPSC-derived alveolar type 2 cells (iAT2s)

Project description:SPC2B2 iAT2s were transduced with either control WT YAP or constitutively active nuclear YAP (YAP5SA) lentivirus in a single cell suspension and were then grown in iAT2 media (CK + DCI) in 3D matrigel for 7 days.

2023-08-08 | GSE221344 | GEO

Tgfb in AT1 cells

Project description:Premature birth disrupts normal lung development and places infants at risk for bronchopulmonary dysplasia (BPD), a disease increasing in incidence which disrupts lung health throughout the lifespan. The TGFβ superfamily has been implicated in BPD pathogenesis, however, what cell lineage it impacts remains unclear. We show that the primary TGFβ receptor, Tgfbr2, is critical for AT1 cell fate maintenance and function. Loss of Tgfbr2 in AT1 cells during late lung development leads to AT1-AT2 cell reprogramming and altered pulmonary architecture, which persists into adulthood. Restriction of fetal lung stretch and associated AT1 cell spreading through a model of oligohydramnios enhances AT1-AT2 reprogramming. Transcriptomic and proteomic analysis reveal that expression of extracellular matrix components by AT1 cells is attenuated with loss of Tgfbr2. Cell spreading assays shows that TGFβ signaling regulates integrin transcription to alter AT1 cell morphology, which further impacts ECM expression through changes in mechanotransduction. These data reveal the cell intrinsic necessity of TGFβ signaling to maintain AT1 fate and define this cell lineage as a major orchestrator of the alveolar matrisome, which, if altered, may predispose to BPD.

2024-03-04 | GSE230268 | GEO

Single Cell sequencing analysis of adult AT1 Yap/Taz KO and wild-type lung epithelium

Project description:The goal of this study was to compare wild-type adult homeostatic lung epithelium to Hopx-creERT2-induced Yap/Taz KO in adult AT1 cells. Three adult mice with identical genotypes and either sex were pooled after fluorescence-activated cell sorting and loaded into the 10X Chromium scRNA sequencing library generation pipeline.

2021-05-20 | GSE164422 | GEO

N-Acetylation of secreted proteins is widespread in Apicomplexa and independent of acetyl-CoA transporter AT1

Project description:Acetyl-CoA critically participates in post-translational modification of proteins, central carbon and lipid metabolism in several compartments of eukaryotic cells. In mammals, the acetyl-CoA transporter 1 (AT1) facilitates the flux of cytosolic acetyl-CoA into the endoplasmic reticulum (ER) enabling the acetylation of proteins of the secretory pathway in concert with dedicated acetyltransferases including Nat8. Homologues of AT1 and Nat8 were identified in the apicomplexan parasites Toxoplasma gondii and Plasmodium berghei. However, the implication of acetyl-CoA pool influx into the ER in acetylation of ER-transiting proteins and their relevance throughout the parasites’ life cycle is unknown. Here, we report proteome-wide analyses, which revealed unprecedented widespread N-terminal acetylation marks of secreted proteins in both parasites. Deletion of the gene coding for AT1 in both parasites, resulted in global loss of fitness and in addition to retardation in erythrocytic development, malaria parasites are blocked in transmission to mosquitoes. However, in absence of AT1 proteome wide lysine and N-terminal acetylation modifications remain unaltered. This highlights a role of AT1 in parasite development uncoupled to acetylation capacity, indicative of an unusually active acetylation machinery occurring in the ER of Apicomplexa.

2022-06-20 | PXD029616 | Pride

Single Cell sequencing analysis of AT1/AT2 cells from wildtype mouse, AT1-Cdc42 knockout mouse, and left bronchial ligation model

Project description:The goal of this study was to compare wildtype adult lung epithelium to epithelium from Cdc42 AT1-KO mice and mice after left bronchial ligation model.

2024-05-01 | GSE202317 | GEO

Characterization of lysine acetylation in Toxoplasma gondii proteome lacking acetyl-CoA transporter 1 (AT1).

Project description:Acetyl-CoA participates in post-translational modification of proteins, central carbon and lipid metabolism in several cell compartments. In mammals, the acetyl-CoA transporter 1 (AT1) facilitates the flux of cytosolic acetyl-CoA into the endoplasmic reticulum (ER), enabling the acetylation of proteins of the secretory pathway, in concert with dedicated acetyltransferases including Nat8. However, the implication of the ER acetyl-CoA pool in acetylation of ER-transiting proteins and their relevance throughout the parasites’ life cycle is unknown. Here, we evaluated the impact of blocking putative cytosolic acetyl-CoA import on acetylation of proteins through KO of the homologue of AT1 in the parasite Toxoplasma gondii.

2022-07-25 | PXD030932 | Pride

Profiling AT1 and AT2 cells throughout alveolar development

Project description:This experiment profiled the transcriptomes of developing AT1 and AT2 cells from the beginning of embryonic alveolar development through the end of alveologenesis at 6 weeks of age.

2021-05-20 | GSE148487 | GEO

Functional and Mechanistic Neurotoxicity Profiling Using Human iPSC-Derived Neural 3D Cultures.

Project description: Not available

| S-EPMC6317428 | biostudies-literature

Single-cell RNA-seq in AT1 cell-ablated mouse lungs

Project description:We performed single-cell RNA-seq analysis using Drop-seq S3 method in mouse lungs following AT1 cell ablation

2023-11-02 | GSE218666 | GEO

Quantitative analysis of YAP effects in podocytes

Project description:Human podocytes were transduced with mouse YAP, with mouse YAP 112A and with empty vector using lentiviral gene transfer. Effects of YAP were measured by quantitative proteomics.

2017-05-02 | PXD005182 | Pride

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