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Effect of ablation of PRDM1 on gene expression in human regulatory T cells

ABSTRACT: To gain an insight into the contribution of BLIMP-1 in human Tregs, we ablated PRDM1 in ex-vivo expaned human Tregs by Crispr/Cas9. Tregs transfected with scramble gRNAs were used as controls. 7 days after transfection, Tregs were rested overnight in media, and were re-stimulated through the TCR and IL2R for 16 hr prior to isolation of RNA for genome-wide RNA-seq. Gene expression profiling analysis was performed using data obtained from RNA-seq of four replicates of PRDM1KO and control cells.

ORGANISM(S): Homo sapiens

PROVIDER: GSE221348 | GEO | 2025/07/23

REPOSITORIES: GEO

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Effect of ablation of CEACAM1 on gene expression in human regulatory T cells

Project description:To study the contribution of CEACAM1 in human Tregs, CEACAM1 was knocked out in ex-vivo expanded human Tregs by Crispr/Cas9. Tregs transfected with scramble gRNAs were used as controls. After electroporation, scramble or CEACAM1KO Tregs were subcultured with IL-2 for 7 days and RNA was isolated for genome-wide RNA-seq. Gene expression profiling analysis was performed using data obtained from RNA-seq of two replicates of CEACAM1KO and control cells.

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Project description:Prdm1

| PRJNA300302 | ENA

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2013-04-02 | E-GEOD-34586 | biostudies-arrayexpress

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