Physiology of Pseudomonas aeruginosa in Biofilms Revealed by Comparative Transcriptomic Analysis.
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ABSTRACT:
ORGANISM(S): Pseudomonas aeruginosa
PROVIDER: GSE22164 | GEO | 2010/11/20
SECONDARY ACCESSION(S): PRJNA127703
REPOSITORIES: GEO
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Project description:Abstract: Transcriptome analysis was applied to characterize the physiological activities of Pseudomonas aeruginosa grown for three days in drip-flow biofilm reactors. Conventional applications of transcriptional profiling often compare two paired data sets that differ in a single experimentally controlled variable. In contrast this study obtained the transcriptome of a single biofilm state, ranked transcript signals to make the priorities of the population manifest, and compared rankings for a priori identified physiological marker genes between the biofilm and published data sets. Two drip flow biofilm conditions with three replicates each: (1) baseline control at 72hrs, (2) no treatment for 12 hours past baseline. Data from these two conditions were pooled
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