Autophagy mutant cells accumulates proteins involved in cell proliferation desiccation and rehydration in Physcomitrium patens

ABSTRACT: Autophagy is the process responsible for degrading cytoplasmic components in lysosomes and vacuoles. It has been widely accepted that autophagy contributes to cellular survival against stress, since autophagy-deficient (atg) mutants are predominantly more sensitive to environmental stress, including nutrient starvation, than their wild-type (WT) counterparts. However, Physcomitrium atg mutants are able to survive from desiccation stress compared with WT under presence of ABA. The molecular mechanism is not understood. In this study, comparative proteomic and microarray expression analysis were performed for WT and atg mutant protonemal colonies of Physcomitrium. Integrated analysis showed that signaling pathways involved in MYB, MAPK were activated, and that protective proteins such as LEA and antioxidant-related proteins were not differentially expressed between WT and atg mutant. This suggests that atg mutant was highly tolerant to desiccation stress by activating cell proliferation during both dehydration and rehydration processes. Also, yeast microarray analysis using publicly available data showed that signaling pathways involved in cell proliferation, such as MYB, were activated in rehydration. This shows that activation of cell proliferation is important for inducing desiccation tolerance. This study has provided new evidence that autophagy is involved in the relationship between desiccation tolerance and cell proliferation, but we have not been able to pinpoint its direct effect. Further work is needed to elucidate the dynamics of proteins in autophagy and induction of desiccation tolerance.

ORGANISM(S): Physcomitrium patens

PROVIDER: GSE222559 | GEO | 2026/04/26

REPOSITORIES: GEO