Dataset Information


Expression data from Saccharomyces cerevisiae Met30 temperature-sensitive strain

ABSTRACT: The target of rapamycin (TOR) plays a central role in eukaryotic cell growth control. With prevalent hyper-activation of the mTOR pathway in human cancers, novel strategies to enhance TOR pathway inhibition are highly desirable. We used a yeast-based high-throughput chemical genetic screen to identify small-molecule enhancers of rapamycin (SMERs) and used whole genome expression analysis to identify their mechanisms of action. Overall design: We incubated Met30 temperature-sensitive yeast strain at either the permissive (room temperature) or non-permissive (35°C) temperature for 1 hour prior to RNA extraction and hybridization on Affymetrix microarrays. Comparison of expression profiles enabled identification of gene-signature characteristic of Met30 inhibition.

INSTRUMENT(S): [Yeast_2] Affymetrix Yeast Genome 2.0 Array

ORGANISM(S): Saccharomyces cerevisiae  

SUBMITTER: Mariam Aghajan 

PROVIDER: GSE22270 | GEO | 2011-06-30



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