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Arabidopsis thaliana ecotype Columbia [Col-0]: incubated with E. coli vs. Control

ABSTRACT: To explore mechanisms involved in the plant-microbe interactions, we proceeded with genome-wide transcriptome analysis of Arabidopsis roots incubated with E. coli Bl21 for 24 hours. Control plants did not receive E. coli.

ORGANISM(S): Arabidopsis thaliana

PROVIDER: GSE22277 | GEO | 2010/12/01

SECONDARY ACCESSION(S): PRJNA128703

REPOSITORIES: GEO

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Arabidopsis thaliana ecotype Columbia [Col-0]: incubated with E. coli vs. Control

Project description:To explore mechanisms involved in the plant-microbe interactions, we proceeded with genome-wide transcriptome analysis of Arabidopsis roots incubated with E. coli Bl21 for 24 hours. Control plants did not receive E. coli. Two-condition experiment, E. coli vs. Control. Biological replicates: 3 E. coli replicates, 3 control replicates

2010-12-01 | E-GEOD-22277 | biostudies-arrayexpress

Arabidopsis thaliana ecotype Columbia [Col-0]): incubated with yeast (saccharomyces cerevisiae) vs. Control

Project description:Transcriptional profiling of Arabidopsis incubated with yeast comparing to control (without yeast incubation).

2017-12-31 | GSE27006 | GEO

Expression data from Escherichia coli

2006-07-07 | GSE5239 | GEO

Transcription profiling of E. coli BL21, BL21/pOri1 and BL21/pOri2mutants

Project description:Transcription profiles in BL21, BL21/pOri1 and BL21/pOri2 were analysed using DNA microarray technology. BL21, BL21/pOri1 or BL21/pOri2 strains were cultured at chemostat status and harvested after the cultivation arrived steady status. Experiment Overall Design: BL21, BL21/pOri1 and BL21/pOri2 were cultured at the exponential growth status or at the same growth rate status, respectively. E. coli BL21, BL21/pOri1 and BL21/pOri2 RNAs were reverse-transcripted into cDNAs, The RNAs from the BL21, BL21/pOri1, BL21/pOri2 were labelled with biotin. The labelled cDNAs were mixed and then hybridized on the microarray slides. Experiments were repeated four times. Most of them have no significant difference comparing plasmid-carrying E. coli and plasmid-free E. coli

2008-06-13 | E-GEOD-5239 | biostudies-arrayexpress

Global transcriptomic analysis of Arabidopsis thaliana ecotype Columbia-0 subjected to magnesium deficiency

Project description:Magnesium (Mg) is essential for many biological processes in plant cells and its deficiency causes yield reduction in crop systems. Low Mg status reportedly impacts on photosynthesis, sucrose partitioning and biomass allocation. However, earlier responses to Mg deficiency are scarcely described. Generally, symptoms of nutrient deficiency appear in specific ages of leaves. Therefore, we hypothesised that transcriptional responses to Mg deficiency are different depending on the ages of leaves, and performed a global transcriptomic analysis in two types of leaves; source and sink leaves of the model plant species Arabidopsis thaliana to reveal the earlier responses to Mg deficiency. The global transcriptomic study revealed that short-term Mg deficiency triggers the expression of defence response genes in sink leaves. In roots, although short-term Mg deficiency enhanced the Mg2+ uptake from the environmnet, transcriptional levels of genes encoding putative Mg2+ transporters in roots were unchanged, suggesting non-transcriptional regulation of Mg2+ uptake in roots.

2020-04-29 | GSE140070 | GEO

E.coli BL21 vs HLT013

Project description:Transcriptional profiling of E. coli strain comparing control BL21 with recombineered HLT013. HLT013 strains was derived from BL21 and capable of producing thymidine.

2015-06-18 | GSE69963 | GEO

Time series of Escherichia coli BL21(DE3) after temperature upshift from 37°C to 42°C, tiling arrays

Project description:Heat-responsive and time-resolved changes in transcriptome of E. coli BL21(DE3) Experimentally mapped transcriptome structure of Escherichia coli BL21(DE3) by hybridizing total RNA (including RNA species <200 nt) to genome-wide high-density tiling arrays (60 mer probes tiled every 10 nt).

2020-04-12 | GSE148034 | GEO

Arabidopsis thaliana ecotype Col-0 versus T-DNA mutant sir1-1

Project description:Arabidopsis thaliana Col-0 plants were compared to sir1-1 T-DNA insertion mutants to investigate transcript levels of sulfur metabolism related genes under standard conditions.

2010-03-12 | GSE20670 | GEO

Protoplast-Esculin Assay as a New Method to Assay Plant Sucrose Transporters: Characterization of AtSUC6 and AtSUC7 Sucrose Uptake Activity in Arabidopsis Col-0 Ecotype.

Project description: Not available

| S-EPMC5925572 | biostudies-literature

2-week-old Arabidopsis seedlings (Columbia ecotype)

Project description:Comparison of TopHat alignments and assessment of spurious splice junctions for 32nt and 76nt read lengths. Total RNA from 2-week-old Arabidopsis thaliana (ecotype Columbia) seedlings grown on MS plates was isolated using RNeasy Plant Mini Kit from Qiagen. To remove any contaminating DNA, RNA was treated with DNAse. Isolation of poly (A) mRNA and preparation of cDNA library were carried out using the Illumina TrueSeq RNA kit. Sequencing (72 cycle) was done on Illumina Genome Analyzer II.

2011-09-24 | GSE32318 | GEO

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