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The active form of Mef2c promotes maturation of induced cardiomyocytes

ABSTRACT: This SuperSeries is composed of the SubSeries listed below.

ORGANISM(S): Mus musculus

PROVIDER: GSE222870 | GEO | 2023/06/13

REPOSITORIES: GEO

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The active form of Mef2c promotes maturation of induced cardiomyocytes

Project description:The active form of Mef2c promotes maturation of induced cardiomyocytes

| PRJNA923681 | ENA

The active form of Mef2c promotes maturation of induced cardiomyocytes with p300 in direct cardiac reprogramming

Project description:Direct cardiac reprogramming converts fibroblasts into induced cardiomyocytes (iCMs) with the minimal combination of transcription factors, Gata4 (G), Mef2c (M), and Tbx5 (T). However, the induction of functional mature iCMs is inefficient and the mechanisms remain elusive. Mef2c is a central transcription factor in direct cardiac reprogramming. We investigated the effect of Mef2c isoforms(M1, M2, M6) and transcriptional activity (M2TAD) on cardiac reprogramming on cardiac reprogramming. Then, we found that the active form of Mef2c evoked epigenetic remodeling cooperating with p300 and promoted the maturation of iCMs.

2023-06-13 | GSE196293 | GEO

MEF2C/p300-mediated epigenetic remodeling promotes the maturation of induced cardiomyocytes.

Project description:Cardiac transcription factors (TFs) directly reprogram fibroblasts into induced cardiomyocytes (iCMs), where MEF2C acts as a pioneer factor with GATA4 and TBX5 (GT). However, the generation of functional and mature iCMs is inefficient, and the molecular mechanisms underlying this process remain largely unknown. Here, we found that the overexpression of transcriptionally activated MEF2C via fusion of the powerful MYOD transactivation domain combined with GT increased the generation of beating iCMs by 30-fold. Activated MEF2C with GT generated iCMs that were transcriptionally, structurally, and functionally more mature than those generated by native MEF2C with GT. Mechanistically, activated MEF2C recruited p300 and multiple cardiogenic TFs to cardiac loci to induce chromatin remodeling. In contrast, p300 inhibition suppressed cardiac gene expression, inhibited iCM maturation, and decreased the beating iCM numbers. Splicing isoforms of MEF2C with similar transcriptional activities did not promote functional iCM generation. Thus, MEF2C/p300-mediated epigenetic remodeling promotes iCM maturation.

| S-EPMC10277823 | biostudies-literature

Mef2c/p300 Promotes Maturation of Induced Cardiomyocytes

Project description:Cardiac transcription factors (TFs) directly reprogram fibroblasts into induced cardiomyocytes (iCMs), where Mef2c acts as a pioneer factor with Gata4 and Tbx5 (GT). However, generation of functional and mature iCMs is inefficient and molecular mechanisms underlying this process remains largely unknown. Here we found that transduction of transcriptionally activated Mef2c via fusion of the powerful MyoD transactivation domain increased generation of beating iCMs by 30-fold in combination with GT.

2023-06-13 | GSE222869 | GEO

Mef2c/p300 Promotes Maturation of Induced Cardiomyocytes

Project description:Mef2c/p300 Promotes Maturation of Induced Cardiomyocytes

| PRJNA923690 | ENA

The active form of Mef2c promotes maturation of induced cardiomyocytes with p300 in direct cardiac reprogramming

Project description:The active form of Mef2c promotes maturation of induced cardiomyocytes with p300 in direct cardiac reprogramming

| PRJNA804194 | ENA

Gene expression profiles of induced cardiomyocytes(iCMs)

Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

2014-07-03 | E-GEOD-56913 | biostudies-arrayexpress

Histone Deacetylase Inhibition Promotes Osteoblast Maturation by Altering the Histone 4 (H4) Epigenome

Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

2013-09-04 | E-GEOD-50552 | biostudies-arrayexpress

Arid1a is essential in cardiomyocytes for neonatal heart maturation

Project description:This SuperSeries is composed of the SubSeries listed below.

2023-07-05 | GSE155102 | GEO

Nicotinamide promotes human pluripotent stem cells differentiation into cardiomyocytes

Project description:This SuperSeries is composed of the SubSeries listed below.

2021-11-30 | GSE154455 | GEO

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