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NGS of phage antibody outputs from whole-cell panning


ABSTRACT: We present a target-unbiased approach for antibody discovery that relies on generating mAbs against native target cell surfaces via phage display. This method combines a previously reported method for improved whole-cell phage display selections with next-generation sequencing analysis to efficiently identify mAbs with the desired target cell reactivity. This approach enabled the identification of three multiple myeloma cell surface antigens, and cognate monoclonal antibody probes.

ORGANISM(S): Oryctolagus cuniculus

PROVIDER: GSE222897 | GEO | 2023/01/18

REPOSITORIES: GEO

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