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Effect of depletion of ADAMTS12 on gene expression in the LX-2 cell line derived from activated human hepatic stellate cells (HSCs)

ABSTRACT: Activated HSCs are liver stromal cells that play an important rôle in tissue repair and fibrosis. We performed gene expression profiling analysis using data obtained from RNA-seq of LX-2 cells treated for 48h with control siRNAs (siCTL) or siRNAs specific for ADAMTS12 (siADAMTS12)

ORGANISM(S): Homo sapiens

PROVIDER: GSE223753 | GEO | 2023/01/29

REPOSITORIES: GEO

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Human hepatic stellate cell line LX-2 activated under acidic culture condition

Project description:We found that the number of tumor-infiltrating myofibroblasts was positively correlated to tumor acidification status in hepatocellular carcinoma (HCC). Hepatic stellate cells (HSCs), the predominant precursors of liver myofibroblasts, were activated and transdifferentiated into myofibroblasts under acidic culture condition. To identify the molecular phenotype of LX-2 cells in acidic culture conditions, we further conducted a gene expression profile analysis. LX-2 cells cultured in pH 7.2 or pH 6.2 medium separately for six days was used in gene expression microarray analysis.

2013-07-29 | E-GEOD-49301 | biostudies-arrayexpress

Human hepatic stellate cell line LX-2 activated under acidic culture condition

2013-07-29 | GSE49301 | GEO

MicroRNA expression profile of human hepatic stellate cells LX-2 co-cultured with human umbilical cord mesenchymal stem cells and control group

Project description:Hepatic stellate cells(HSCs) are the main effector cells of liver fibrosis. In order to study the effect of mesenchymal stem cells(MSCs) on microRNAs expression of HSCs, we co-cultured HSCs LX-2 activated by TGFβ1 with human umbilical cord MSCs(hUC-MSCs) for 48 hours, and compared the differentially expressed miRNA with LX-2 cultured alone by high-throughput sequencing. The results showed that two mature microRNAs expressed increased, and nine expressed decreased.

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RNA sequencing of the hepatic stellate cell line LX-2 treated with either DMSO or Aramchol after 24 or 48 hours.

Project description:To investigate whether Aramchol might reduce liver fibrosis in part directly inhibiting HSCs, we treated LX-2 cells with Aramchol, 10 µM for 24 or 48 hours, then performed RNA sequencing. Sequencing libraries were made with the TruSeq Stranded mRNA Library Prep Kit by manufacturers suggestions. Sequencing parameters are as follows: poly-A RNA enrichment, 175 bp paired reads ran on HiSeq2500 Illumina instrument. Mapping of raw sequencing reads was performed using STAR (2.4.0c) to the UCSC GRCh37/hg19 reference genome.

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Transcriptome of human LX-2 hepatic stellate cells transfected with siRNA directed against OGT

Project description:RNA-seq analysis of human LX-2 cells treated transfected or not with siOGT.

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Investigation of hepatic stellate cell proteomics during activa-tion with fetal bovine serum

Project description:Hepatic stellate cells (HSCs) are the major driving factor in liver fibrosis. Upon liver inflammation caused by alcohol abuse and/or a fatty liver, HSCs transform from a quiescent- into a proliferating, fibrotic phenotype. We study this transformation termed “activation”, using state of the art TIMS-TOF mass spectrometry proteomics, as well as phenotype analysis of the immortalized LX-2 HSC cell line. In our work we employ a simple, yet reliable model of HSC activation via an in-crease in growth media serum concentration (serum activation). Protein network analysis of ac-tivated LX 2 cells reveals an increase in the production of ribosomal proteins and proteins related to migration. Interestingly, we could also observe a decrease in the expression of lipogenic pro-teins and a loss of cytosolic lipid droplets during activation. Especially the downregulation of proteins associated with cholesterol biosynthesis might be a promising target for further study. This work provides an update on HSC activation characteristics using contemporary proteomic and bioinformatic analysis and presents an accessible model for HSC activation.

2022-02-14 | PXD029121 | Pride

O-GlcNAcylation controls pro-fibrotic transcriptional regulatory signaling in hepatic stellate cells (HSCs)

Project description:Human LX-2 cells were used for TEAD4 ChIP-seq

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Effect of OGT inhibition on the epigenome of human LX-2 hepatic stellate cells

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2024-05-22 | GSE243103 | GEO

Transcriptome of human LX-2 hepatic stellate cells treated with the OGT inhibitor OSMI-1

Project description:RNA-seq analysis of human LX-2 cells treated or not with OSMI-1 OGT inhibitor (OGTi).

2024-05-22 | GSE243102 | GEO

RNA-seq analysis of the gene expression profile of Huh7 cells upon incubation with different TGF-β-treated hepatic stellate cells (HSCs)-derived ectosomes

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2024-04-28 | GSE173632 | GEO

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