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Genome-wide Analysis of Enterococcus faecalis OG1RF Gene Expression During Infection

ABSTRACT: Changes in Enterococcus faecalis OG1RF gene expression during infection in a rabbit model of subdermal abscess formation were studied using microarray analysis.

ORGANISM(S): Enterococcus faecalis V583 Enterococcus faecalis OG1RF

PROVIDER: GSE22391 | GEO | 2013/12/15

SECONDARY ACCESSION(S): PRJNA127627

REPOSITORIES: GEO

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Project description:Changes in Enterococcus faecalis OG1RF gene expression during infection in a rabbit model of subdermal abscess formation were studied using microarray analysis. Samples assayed in arrays consisted of the input inoculum and total RNA collected from the subdermal chambers at 2 and 8 hours post-inoculation. Alexa647-labeled samples were co-hybridized with 0.5 micrograms of sheared OG1RF genomic DNA labeled with Cy3 as a standard reference between chips. Four biological replicates were performed for each of the 3 time points.

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