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Plucked scalp hair follicle samples are useful RNA sources for mRNA analysis of genodermatosis-associated genes

ABSTRACT: Using RNA samples extracted from plucked hair follicles and skin samples, we conducted comprehensive RNA sequencing and evaluated expression levels of 347 genes causative of genodermatoses.

ORGANISM(S): Homo sapiens

PROVIDER: GSE224010 | GEO | 2023/06/19

REPOSITORIES: GEO

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Transcription profiling of human hair follicles from men and women

Project description:Investigation of Gene Expression Profiling in Unstaged Head Hair Follicles Plucked from Men and Women Experiment Overall Design: Total RNA was extracted from 1-3 unstaged follicles plucked from the scalp of each of 36 adult human volunteers. The average quantifiable yield of RNA per follicle was 112.5 ng. Ten samples were selected, based on ribosomal ratio, relative integrity number and total yield, for expression profiling. By preamplifying the extracted RNA (starting with as little as 30 ng), sufficient labeled RNA was generated to conduct Afymetrix-based gene expression analysis.

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Gene Expression in Unstaged Head Hair Follicles Plucked from Men and Women

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Bald scalp retains hair follicle stem cells but lacks CD200-rich and CD34-positive hair follicle progenitor cells

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mRNA profiling of human plucked hair follicle

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Bald scalp in men with androgenetic alopecia retains hair follicle stem cells but lacks CD200-rich and CD34-positive hair follicle progenitor cells.

Project description: Not available

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Expression data from human hair follicle after doxorubicin treatment

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