Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

ST105-01: UNBIASED IN-VIVO AND IN-SILICO TL-532 MECANISM OF ACTION BY RNA-SEQUENCING

ABSTRACT: Tollys characterized and patented a family of TLR3 proprietary ligands, including the drug candidate TL-532 considered in this study. The aim of this project is to use unbiassed high-throughput transcriptomic approach to decipher signaling pathways linked to the mechanisms of action of the drug candidate TL-532 (including induction of programmed death, immunological activation, TH1 and BDCA3 response, cytokines and chemokines expression, angiogenesis, infiltrates, etc.), induced early (3h, 6h and 24h post-treatment), after a single locoregional treatment, at a dose known to be very effective in this model.

ORGANISM(S): Mus musculus

PROVIDER: GSE224041 | GEO | 2024/05/31

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

ST105-01: UNBIASED IN-VIVO AND IN-SILICO TL-532 MECANISM OF ACTION BY RNA-SEQUENCING

Project description:ST105-01: UNBIASED IN-VIVO AND IN-SILICO TL-532 MECANISM OF ACTION BY RNA-SEQUENCING

| PRJNA929475 | ENA

Coemansia sp. RSA 532 isolate:RSA 532

Project description:Coemansia sp. RSA 532 Resequencing

| PRJNA519294 | ENA

Sinorhizobium medicae strain:532

Project description:Sinorhizobium medicae 532 Resequencing

| PRJNA499444 | ENA

H3K27ac HiChIP analysis in TL-Om1 cells

Project description:H3K27ac HiChIP analysis was performed in an adult T-cell leukemia/lymphoma cell line (TL-Om1) to analyze active chromatin-chromatin interactions in TL-Om1 cells.

2023-11-20 | GSE181214 | GEO

IRF4 ChIP-seq analysis in TL-Om1 cells

Project description:ChIP-seq analysis was performed in an adult T-cell leukemia/lymphoma cell line (TL-Om1) to analyze DNA bindings of IRF4 in TL-Om1 cells.

2020-02-03 | GSE116763 | GEO

Streptococcus sp. G09-532

Project description:Streptococcus sp. G09-532 Genome sequencing

| PRJNA1246268 | ENA

ChIP-seq analysis for p65 protein in TL-Om1 cells

Project description:ChIP-seq analysis was performed in an adult T-cell leukemia/lymphoma cell line (TL-Om1) to analyze DNA bindings of p65 in TL-Om1 cells.

2020-02-03 | GSE133006 | GEO

ChIP-seq analysis for TP73 protein in TL-Om1 cells

Project description:ChIP-seq analysis was performed in an adult T-cell leukemia/lymphoma cell line (TL-Om1) to analyze DNA bindings of TP73 in TL-Om1 cells.

2023-11-20 | GSE181213 | GEO

TTF-1-regulated miR-532-5p targets KRAS and MKL2 oncogenes and induces apoptosis in lung adenocarcinoma [pts]

Project description:Thyroid transcription factor-1 (TTF-1), also known as NKX2-1, plays a role as a lineage-survival oncogene in lung adenocarcinoma with double-edged sword characteristics. Although previous studies steadily accumulated evidence for roles of TTF-1 in the transcriptional regulation of protein-coding genes, very little is known about its regulatory relationship with miRNAs. In this study, we have identified miR-532-5p as a novel transcriptional target of TTF-1 by an integrative approach, which was designed to extract maximal information from expression profiles of both patient tumors in vivo and TTF-1-inducible cell lines in vitro. Consequently, we have found that miR-532-5p is directly regulated by TTF-1 through its binding to a genomic region 8 kb upstream of miR-532-5p, which appeared to impose transcriptional regulation independent of that of CLCN5, a protein-coding gene harboring miR-532-5p in its intron 3. Further, we have also identified KRAS and MKL2 as novel direct targets of miR-532-5p. Introduction of miR-532-5p mimics markedly induced apoptosis in KRAS-mutant as well as KRAS wildtype lung adenocarcinoma cell lines. Interestingly, miR-532-5p affected the MEK-ERK pathway signaling specifically in cell lines sensitive to siKRAS treatment, while the miR-532-5p-mediated effects were clearly phenocopied by repressing expression or inhibiting function of MKL2 regardless of KRAS mutation status. In summary, our findings demonstrate that miR-532-5p is as novel transcriptional target of TTF-1 and plays a tumor suppressive role by targeting KRAS and MKL2 in lung adenocarcinoma. Novel therapeutic strategies using miR-532-5p or an MKL2 inhibitor may prove effective against this hard-to-cure cancer irrespective of the dependence on KRAS-mediated signaling.

2017-06-21 | GSE83836 | GEO

TTF-1-regulated miR-532-5p targets KRAS and MKL2 oncogenes and induces apoptosis in lung adenocarcinoma [rt-pcr]

2017-06-21 | GSE83838 | GEO