Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Effects of USB1 mutation on global miRNome during hESC-derived definitive hematopoiesis

ABSTRACT: USB1 mutations lead to poikiloderma with neutropenia. We investigated the effect of inactivating USB1 on the global miRome in undifferentiated and differentiated hematopoietic stem cells

ORGANISM(S): Homo sapiens

PROVIDER: GSE224431 | GEO | 2023/02/07

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

Effect of USB1 mutation on global transcriptome during hESC-derived definitive hematopoiesis

Project description:USB1 mutations lead to poikiloderma with neutropenia. We investigated the effect of inactivating USB1 on the global transcriptome in undifferentiated hESCs and differentiated hematopoietic progeny

2023-02-07 | GSE224432 | GEO

Effects of USB1 knockout on global miRNome in K562 cells

Project description:USB1 mutations lead to poikiloderma with neutropenia. We investigated the effect of inactivating USB1 on the global miRome in K562 cells

2023-02-07 | GSE224430 | GEO

Effect of USB1 depletion on global miRNome in human embryonic stem cells and K562

Project description:USB1 mutations lead to poikiloderma with neutropenia. We investigated the effect of inactivating USB1 on the global miRNome in undifferentiated hESCs and K562

2024-02-05 | GSE224501 | GEO

Mutation of kri1l causes definitive hematopoiesis failure via PERK-dependent excessive autophagy induction.

Project description: Not available

| S-EPMC4528055 | biostudies-literature

A germline point mutation in Runx1 uncouples its role in definitive hematopoiesis from differentiation.

Project description: Not available

| S-EPMC3909992 | biostudies-literature

Transcription profiling by high throughput sequencing of MPN1 deficient human cells

Project description:Clericuzio-type poikiloderma with neutropenia (PN, Mendelian Inheritance in Man (MIM) accession 604173) is a rare genodermatosis associated to mutations in the MPN1 gene. The aim of this experiment was to investigate the the effect of MPN1 mutations on pre-mRNA transcripts maturation. We compared the transcriptome obtained from 2 lymphoblastoid immortal cell lines from PN patients with the one of a lymphoblastoid cell line established from the healthy brother of one of the patient. The trasncriptome was generated by single-end RNA-Seq.

2012-07-27 | E-MTAB-1197 | biostudies-arrayexpress

Sustained intrinsic WNT and BMP4 activation impairs hESC differentiation to definitive endoderm and drives the cells towards extra-embryonic mesoderm

Project description:This dataset includes the RNA sequencing of five replicates of the undifferentiated hESC subline VUB03_S2, which shows impaired mesendoderm induction, and 14 WT samples from GSE116372.

2021-04-06 | GSE134454 | GEO

Endoglin potentiates nitric oxide synthesis to enhance definitive hematopoiesis.

Project description: Not available

| S-EPMC4571086 | biostudies-literature

Characterization of Definitive Endoderm formation from HESC and iPSC lines by Microarray analysis

Project description:HESC-H9 and iPSC lines 3.5, 3.6 and 3.12 were analyzed using Affymetrix microarray before and after Definitive Endoderm (DE) formation. DE was induced using the ActivinA differentiation protocol described by D'Amour et al., 2006 (PMID: 16258519) Clustering analysis of transcripts that were differentially regulated during DE formation indicated that iPSC lines 3.5 and 3.12 differentiate in manner that is highly similar to HESC-H9 cells iPSC line 3.6 had a more divergent transcriptional profile. Three induced pluripotent stem cell lines (iPSC) and one human embryonic stem cell line (hESC - H9) were collected as undifferentiated (UD) cells, and flash frozen. These cell lines were also subjected to definitive endoderm (DE) induction, collected and flash frozen. RNA was harvested from the frozen cell pellets and hybridized to the Affymetrix microarray chip. The three iPSC cell lines are iPSC 3.5, iPSC 3.6. and iPSC 3.12. In the UD state, iPSC 3.5 was analyzed in duplicate, while iPSCs 3.6 and 3.12 and H9s were analyzed in biological triplicate. All four cell lines were analyzed as biological triplicates for DE induction.

2010-12-25 | E-GEOD-25557 | biostudies-arrayexpress

Stable multilineage xenogeneic replacement of definitive hematopoiesis in adult zebrafish.

Project description: Not available

| S-EPMC4726038 | biostudies-literature