Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Acclimation Effects on Acute Thermal Stress in the goby, Gillichthys mirabilis

ABSTRACT: The goal of this study was to identify transcriptional resonses to acute heat stress following differential acclimation in the highly eurythermal goby fish, Gillichthys mirabilis. G. mirabilis were acclimated to 3 temperatures (9C, 19C and 28C) for one month and then exposed to an acute heat ramp at 4C/hr and sampled during the timecourse. Gill tissues were dissected and immediately flash frozen in liquid nitrogen. Total RNA was extracted, reverse transcribed into cDNA, labeled with cy5 and hybridized against a common reference (cy3) on 2-color cDNA microarray developed for this species. Significant genes were determined by expression differences between groups (one-way ANOVA, FDR<0.05) .

ORGANISM(S): Gillichthys mirabilis

PROVIDER: GSE22462 | GEO | 2010/09/15

SECONDARY ACCESSION(S): PRJNA128625

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

Acclimation Effects on Acute Thermal Stress in the goby, Gillichthys mirabilis

Project description:The goal of this study was to identify transcriptional resonses to acute heat stress following differential acclimation in the highly eurythermal goby fish, Gillichthys mirabilis. G. mirabilis were acclimated to 3 temperatures (9C, 19C and 28C) for one month and then exposed to an acute heat ramp at 4C/hr and sampled during the timecourse. Gill tissues were dissected and immediately flash frozen in liquid nitrogen. Total RNA was extracted, reverse transcribed into cDNA, labeled with cy5 and hybridized against a common reference (cy3) on 2-color cDNA microarray developed for this species. Significant genes were determined by expression differences between groups (one-way ANOVA, FDR<0.05) . Common reference design

2010-09-15 | E-GEOD-22462 | biostudies-arrayexpress

Steady State Thermal Acclimation in the Eurythermal Goby fish, Gillichthys mirabilis

Project description:The goal of this study was to identify transcriptional resonses to thermal acclimation in a highly eurythermal fish. Specifically, what are the genes necessary to maintain homostasis at different temperatures and do these genes underlie any known acclimatory responses? Gillichthys mirabilis (goby fish) were acclimated to 3 temperatures (9C, 19C and 28C) for one month. Gill tissue from 7-9 individuals were dissected and immediately flash frozen in liquid nitrogen. Total RNA was extracted, reverse transcribed into cDNA, labeled with cy5 and hybridized against a common reference (cy3) on 2-color cDNA microarray developed for this species. Significant genes were determined by expression differences between groups (one-way ANOVA, FDR<0.05) .

2010-07-15 | GSE20849 | GEO

Steady State Thermal Acclimation in the Eurythermal Goby fish, Gillichthys mirabilis

2010-07-15 | E-GEOD-20849 | biostudies-arrayexpress

Heat stress response in Gillichthys mirabilis

Project description:The goal of this study was to measure the effect of heat stress on the transcriptome of a temperate fish species - Gillichthys mirabilis. Keywords: Stress response

2007-12-14 | GSE9870 | GEO

Gene expression during hyper and hypoosmotic stress adaptation in Gillichthys mirabilis

Project description:This study investigates changes in gene expression associated with adaptation to acute hyper- and hypoosmotic stress in Gillichthys mirabilis gill tissue. Gene expression data was captured as a time course (1, 2, 4, 12 hours past exposure) during the first 12 hours following abrupt transfer to osmotic stress media. We employed a custom made Gillichthys mirabilis cDNA microarray. A total of 39 arrays were used in this study: 12 hyperosmotic stress samples, 12 hypoosmotic stress samples, 12 control samples, and 3 time=0 samples. Three individuals were hybridized at each of the four time points (1, 2, 4, 12 hours past exposure) for experimental and control fish (giving a total of 12). All samples were hybridized against a common reference sample containing pooled cDNAs from several individuals.

2008-06-24 | E-GEOD-11700 | biostudies-arrayexpress

Gillichthys mirabilis

Project description:Heat stress response in Gillichthys mirabilis

| PRJNA105133 | ENA

Gene expression during hyper and hypoosmotic stress adaptation in Gillichthys mirabilis

2008-06-24 | GSE11700 | GEO

Gillichthys mirabilis

Project description:Gillichthys mirabilis overview

| PRJNA108711 | ENA

Gillichthys mirabilis

Project description:Acclimation Effects on Acute Thermal Stress in the goby, Gillichthys mirabilis

| PRJNA128625 | ENA

Gene expression in the Antarctic fish Trematomus bernacchii shows a muted response to heat stress

Project description:The goal of this study was to measure the effect of heat stress on the transcriptome of a cold-adapted fish (Trematomus bernacchii) and compare it to a temperate species (Gillichthys mirabilis). This SuperSeries is composed of the SubSeries listed below.

2007-12-14 | GSE9872 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data