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Hormone-Independence of Prostate Cancer Cells is Supported by the Androgen Receptor without Binding to Classical Response Elements

ABSTRACT: Treatment of late passage (LP50) LNCaP cells with R1881 (androgen) and AR shRNA identified a gene program controlled by androgen receptor in the absence of androgen.

ORGANISM(S): Homo sapiens

PROVIDER: GSE22483 | GEO | 2011/01/28

SECONDARY ACCESSION(S): PRJNA128695

REPOSITORIES: GEO

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Hormone-Independence of Prostate Cancer Cells is Supported by the Androgen Receptor without Binding to Classical Response Elements

Project description:Treatment of late passage (LP50) LNCaP cells with R1881 (androgen) and AR shRNA identified a gene program controlled by androgen receptor in the absence of androgen. Gene expression in late passage (LP50) LNCaP cells that had enhanced androgen-independent growth was determined in androgen-depleted medium in response to R1881 or AR knock down via AR shRNA

2011-01-28 | E-GEOD-22483 | biostudies-arrayexpress

Androgen receptor-coregulators hormone responsiveness LNCaP / GSF

Project description:The text description is: LNCaP are androgen receptor expressing prostate carcinoma cells. Genital skin fibroblasts also express the androgen receptor. Cells were either proliferating or they were G0-arrested. Treatment of cells was performed with either the androgen DHT (dihydrotestosterone), the androgen analogue R1881 (methyltrienolone), or the solvent ethanol. Some hybridizations were performed in the type 1 design. In these cases, the hormone treated sample was hybridized against the ethanol treated sample on the same microarray. Hormone mediated induction or repression of gene transcription can directly be deduced from R/G normalized ratios on arrays. For other experiments, the ethanol treated control and the hormone treated experiment were hybridized on separate microarrays against a common reference of RNA. This reference was composed out of common reference batch CRG (50%) and a fibroblast RNA (50%). This reference was called mixed reference. For all experiments, the same batch of mixed reference was used. A compound treatment design type is where the response to administration of a compound or chemical (including biological compounds such as hormones) is assayed. Compound Based Treatment: Ethanol, R1881 (methyltrienolone), or DHT (dihydrotestosterone) Cell Line: LNCaP (prostate cancer) or foreskin fibroblasts Culture Synchrony: proliferation / Go-arrest Computed

2010-07-01 | E-GEOD-4454 | biostudies-arrayexpress

Androgen receptor-coregulators hormone responsiveness LNCaP / GSF

2006-10-12 | GSE4454 | GEO

Identification of direct androgen-target genes in the prostate cancer cell line LNCaP

Project description:Transcriptional profiling of the LNCaP cell line treated with R1881 synthetic androgen and/or cycloheximide.

2014-06-14 | GSE22372 | GEO

Long-term androgen withdrawal and treatment (R1881) of LNCaP-FGC prostate cancer cells

Project description:LNCaP cells were cultures in steroid depleted medium for 5 days before treatment with synthetic androgen (R1881, 10nM) for 16h. Transcriptomics analysis was performed to compare gene expression changes induced by androgen withdrawal or androgen treatment. Genome-wide transcriptomic analysis of LNCaP cells grown in steroid depleted medium, normal (steroid-containing) medium and R1881 treated cells was performed using the Agilent platform

2013-10-22 | E-GEOD-51460 | biostudies-arrayexpress

Long-term androgen withdrawal and treatment (R1881) of LNCaP-FGC prostate cancer cells

2013-10-22 | GSE51460 | GEO

Expression data from androgen treated LNCaP cells

Project description:Androgens are required for the development of normal prostate, and they are also linked to the development of prostate cancer. We used microarrays to understand the role of androgen in an androgen dependent, androgen receptor (AR) positive human metastatic cell line, LNCaP. LNCaP cells were grown in RPMI medium and they were subjected to stay in phenol-red free, RPMI with charcoal stripped serum for 48h. Synthetic androgen R1881 was added and the cells were allowed to grow for 48h. Control cells were given with corresponding amount of ethanol as vehicle which is used for the solubilization of R1881. Cells were harvested and RNA was isolated for microarray analysis.

2009-12-29 | E-GEOD-17044 | biostudies-arrayexpress

Prostasphere-forming cells of human prostate cancer cell LNCaP: Control vs. Hormone treated

Project description:Transcriptional profiling of LNCaP prostasphere-forming cells, comparing control untreated sphere-forming cells with hormone treated sphere-forming cells. Both estrogen (estradiol) and androgen (R1881) promote the sphere formation of human prostate cancer cell LNCaP. Goal was to determine the effects of hormones on global gene expression of LNCaP sphere-forming cells. Five samples were analyzed. Control (ethanol 1hr), estradiol 1 hour, estradiol 24 hour, R1881 1 hour, R1881 24 hour.

2012-04-23 | E-GEOD-37531 | biostudies-arrayexpress

Androgen arrays for Resveratrol-Induced Gene Expression Profiles in Human Prostate Cancer

Project description:Arrays for Expression levels of androgen-responsive genes in LNCaP cells exposed to dihydrotestosterone, R1881. Gene-expression changes in LNCaP treated with 1 nmol/L R1881 at 7, 9, 18, 24, 50, and 72 hours; 10 nmol/L dihydrotestosterone at 18 and 50 hours; 100 nmol/L dihydrotestosterone and 1 5mol/L dihydrotestosterone at 24 hours; and androgen deprivation at 46 and 70 hours. This set corresponds to Figure 2 of the publication. An all pairs experiment design type is where all labeled extracts are compared to every other labeled extract. Keywords: all_pairs

2006-02-25 | GSE4300 | GEO

Prostasphere-forming cells of human prostate cancer cell LNCaP: Control vs. Hormone treated

2012-04-24 | GSE37531 | GEO

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