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ChIP-chip of Geobacter sulfurreducens PCA with antibody against RNAP and RpoD under various conditions

ABSTRACT: We applied a ChIP-chip approach to elucidate the binding profiles of RNAP and RpoD experimentally under different growth conditions. This technique localizes DNA fragments within DNA-protein complexes enriched by chromatin immunoprecipitation using high-density oligonucleotide tilling arrays.

ORGANISM(S): Geobacter sulfurreducens PCA

PROVIDER: GSE22503 | GEO | 2010/07/01

SECONDARY ACCESSION(S): PRJNA129083

REPOSITORIES: GEO

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ChIP-chip of Geobacter sulfurreducens PCA with antibody against RNAP and RpoD under various conditions

Project description:We applied a ChIP-chip approach to elucidate the binding profiles of RNAP and RpoD experimentally under different growth conditions. This technique localizes DNA fragments within DNA-protein complexes enriched by chromatin immunoprecipitation using high-density oligonucleotide tilling arrays. A 21 ChIP-chip study using immunoprecipitated DNA (IP-DNA) from three culture conditions for RNAP and four culture conditions for RpoD. The high-density oligonucleotide tiling arrays used consisted of 381,174 oligonucleotide probes spaced 20 bp apart (30-bp overlap between two probes) across the G. sulfurreducens genome (NimbleGen). Experiments were conducted as three bioliogical replicates (different cultures).

2010-07-01 | E-GEOD-22503 | biostudies-arrayexpress

Geobacter sulfurreducens PCA

Project description:ChIP-chip of Geobacter sulfurreducens PCA with antibody against RNAP and RpoD under various conditions

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ChIP-chip of Geobacter sulfurreducens PCA with antibody against RpoN under various conditions.

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ChIP-chip of Geobacter sulfurreducens PCA with antibody against various sigma factors under various conditions

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ChIP-chip of Geobacter sulfurreducens PCA with antibody against RpoN under various conditions.

Project description:We applied a ChIP-chip approach to elucidate the σN regulon experimentally under different growth conditions. This technique localizes DNA fragments within σN complexes enriched by chromatin immunoprecipitation using high-density oligonucleotide tilling arrays. A nine ChIP-chip study using immunoprecipitated DNA (IP-DNA) from three separate culture conditions. The high-density oligonucleotide tiling arrays used were consisted of 381,174 oligonucleotide probes spaced 20 bp apart (30-bp overlap between two probes) across the G.sulfurreducens genome (NimbleGen). Experiments were conducted as three bioliogical replicates (different cultures)

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ChIP-chip of Geobacter sulfurreducens PCA with antibody against various sigma factors under various conditions

Project description:We applied a ChIP-chip approach to elucidate the binding profiles of sigma factors (RpH, RpoN and RpoS) experimentally under different growth conditions. This technique localizes DNA fragments within DNA-protein complexes enriched by chromatin immunoprecipitation using high-density oligonucleotide tilling arrays. A 10 ChIP-chip study using immunoprecipitated DNA (IP-DNA) from three culture condtions against three alternative sigma factors . The high-density oligonucleotide tiling arrays used were consisted of 381,174 oligonucleotide probes spaced 20 bp apart (30-bp overlap between two probes) across the G.sulfurreducens genome (NimbleGen). Experiments were conducted as three bioliogical replicates (different cultures).

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Analysis of Geobacter sulfurreducens under multiple growth conditions

Project description:This SuperSeries is composed of the following subset Series: GSE22497: Transcriptome analysis of Geobacter sulfurreducens under multiple growth conditions GSE22503: ChIP-chip of Geobacter sulfurreducens PCA with antibody against RNAP and RpoD under various conditions GSE22511: Genome-wide transcription start site determination of Geobacter sulfurreducens under multiple growth conditions Refer to individual Series

2010-06-30 | E-GEOD-22512 | biostudies-arrayexpress

ChIP-chip analysis of RNAP and Sigma factor binding to the Salmonella enterica serovar Typhimurium chromosome

Project description:ChIP-chip analysis of RNA Polymerase (RNAP), RpoD, RpoE, RpoH and RpoN in exponential phase (OD 0.2) and early stationary phase (OD 2.0) Salmonella enterica serovar Typhimurium SL1344 cultures

2017-04-01 | GSE97283 | GEO

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