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NAT10-mediated ac4C modification promotes neuroectoderm specification of human embryonic stem cells via acetylating NR2F1 mRNA to enhance translation efficiency [acRIP-seq]

ABSTRACT: We use acRIP-seq to report the most comprehensive survey to date of mRNA in human embryonic stem cells (hESCs), neuroepithelial progenitors cells (NEP) and mesendoderm cells (ME)

ORGANISM(S): Homo sapiens

PROVIDER: GSE225049 | GEO | 2025/11/30

REPOSITORIES: GEO

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NAT10-mediated ac4C modification promotes neuroectoderm specification of human embryonic stem cells via acetylating NR2F1 mRNA to enhance translation efficiency [Ribo-seq]

Project description:We use Ribo-seq to report the most comprehensive survey to date of mRNA translation in human embryonic stem cells (hESCs), neuroepithelial progenitors cells(NEP) and mesendoderm cells(ME)

2025-11-30 | GSE225051 | GEO

NAT10-mediated ac4C modification promotes neuroectoderm specification of human embryonic stem cells via acetylating NR2F1 mRNA to enhance translation efficiency

Project description:This SuperSeries is composed of the SubSeries listed below.

2025-11-30 | GSE225052 | GEO

NAT10-mediated ac4C modification promotes neuroectoderm specification of human embryonic stem cells via acetylating NR2F1 mRNA to enhance translation efficiency [acRIP-seq]

Project description:NAT10-mediated ac4C modification promotes neuroectoderm specification of human embryonic stem cells via acetylating NR2F1 mRNA to enhance translation efficiency [acRIP-seq]

| PRJNA934559 | ENA

NAT10-mediated ac4C modification promotes neuroectoderm specification of human embryonic stem cells via acetylating NR2F1 mRNA to enhance translation efficiency [RNA-seq]

Project description:The expression profile data was obtained by RNA-Seq data.

2025-11-30 | GSE225050 | GEO

acRIP-seq to identify NAT10 mediates mRNA ac4C modification in colorectal cancer

Project description:acRIP-seq to identify NAT10 mediates mRNA ac4C modification in colorectal cancer

| PRJNA837441 | ENA

NAT10-mediated ac<sup>4</sup>C modification promotes ectoderm differentiation of human embryonic stem cells via acetylating NR2F1 mRNA.

Project description:Cell fate determination in mammalian development is complex and precisely controlled and accumulating evidence indicates that epigenetic mechanisms are crucially involved. N4-acetylcytidine (ac4C) is a recently identified modification of messenger RNA (mRNA); however, its functions are still elusive in mammalian. Here, we show that N-acetyltransferase 10 (NAT10)-mediated ac4C modification promotes ectoderm differentiation of human embryonic stem cells (hESCs) by acetylating nuclear receptor subfamily 2 group F member 1 (NR2F1) mRNA to enhance translation efficiency (TE). Acetylated RNA immunoprecipitation sequencing (acRIP-seq) revealed that levels of ac4C modification were higher in ectodermal neuroepithelial progenitor (NEP) cells than in hESCs or mesoendoderm cells. In addition, integrated analysis of acRIP-seq and ribosome profiling sequencing revealed that NAT10 catalysed ac4C modification to improve TE in NEP cells. RIP-qRT-PCR analysis identified an interaction between NAT10 and NR2F1 mRNA in NEP cells and NR2F1 accelerated the nucleus-to-cytoplasm translocation of yes-associated protein 1, which contributed to ectodermal differentiation of hESCs. Collectively, these findings point out the novel regulatory role of ac4C modification in the early ectodermal differentiation of hESCs and will provide a new strategy for the treatment of neuroectodermal defects diseases.

| S-EPMC10984107 | biostudies-literature

ac4C RIP-seq analysis of AGS cells upon NAT10 knockout.

Project description:N4-acetylcytidine (ac4C), a conserved chemical modification in eukaryotic prokaryotes that is catalyzed by the N-acetyltransferase 10 (NAT10) enzyme, plays a crucial role in promoting mRNA stability and translation. However, the biological function and mechanisms of NAT10-mediated ac4C in human cancer were poorly defined. In order to investigate the regulatory mechanism of NAT10 in gastric cancer, we performed ac4C RIP-seq(acRIP-seq) analysis in AGS cells with NAT10 knockout compared with control in two repeats.

2024-07-07 | GSE180494 | GEO

acRIP-seq to identify NAT10 mediates mRNA ac4C modification in colorectal cancer

Project description:We performedacRIP-seq between control CRC cells and CRC cells with NAT10 knockdown to identify NAT10 mediates mRNA ac4C modification

2022-08-19 | GSE202826 | GEO

RNA-seq and acRIP-seq in NAT10-knockdown PDAC cells

Project description:RNA-seq and acRIP-seq in NAT10-knockdown PDAC cells

| PRJNA771546 | ENA

NAT10 promotes the progression of clear cell renal cell carcinoma by regulating ac4C acetylation of NFE2L3 and activating AKT/GSK3β signaling pathway

Project description:To determine the role of NAT10 in promoting the progression of ccRCC, we performed RNA sequencing (RNA-Seq) and ac4C-modified RNA immunoprecipitation sequencing (acRIP-seq) on four pairs of ccRCC tissues and their adjacent tissues. A comprehensive analysis of acRIP-seq and RNA-seq showed that the expression and acetylation levels of 199 transcripts were increased . We selected 11 candidate genes of interest and analyzed their acetylation levels. Since ac4C modification can improve mRNA stability, the 11 candidate genes were verified, and the results showed that only NFE2L3 mRNA levels decreased in 786-O cells and A498 cells after NAT10 stable knockdown , so we decided to study the mechanism of action of NFE2L3 in ccRCC.

2023-03-18 | GSE226863 | GEO

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