Trypanosoma brucei infection triggers a broad innate inflammatory and antigenic presentation response in astroglial cells derived from human brain organoids
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ABSTRACT: Here, we described the combined application of human brain organoids and single cell transcriptomics analysis to identify a subpopulation of astroglia cells that upregulate a group of genes in response to the human pathogen Trypanosoma brucei gambiense. Our results identified a population of astroglia that specifically respond to T. brucei by upregulating genes associated with antigen presentation, gliogenesis, and innate immunity. Our data demonstrate that these in vitro-derived human brain organoids are able to sense and respond to T. brucei, and open new avenues to further understand the mechanisms underlying pathogen sensing. This method not only provides an important resource for understanding human tissue responses to infection, but also for refining future studies using either well established cell lines or primary cultures, limiting the number of animals required to study brain infection by African trypanosomes, which is advantageous in the context of 3Rs and ethics in animal research.
ORGANISM(S): Homo sapiens
PROVIDER: GSE225147 | GEO | 2024/02/29
REPOSITORIES: GEO
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