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The impact of substrate stiffness on morphological, transcriptional and functional aspects in RPE [miRNA-Seq]

ABSTRACT: To investigate the influence of stiffness as a major Bruch's membrane characteristic on the RPE transcriptome and morphology. ARPE-19 cells were plated on soft or stiff polyacrylamide gels (PA gels) or standard tissue culture plastic (TCP). We then performed gene expression profiling analysis using data obtained from Next-generation sequencing of small RNAs of ARPE-19 cells cultured on 3 different biomechanical conditions.

ORGANISM(S): Homo sapiens

PROVIDER: GSE225641 | GEO | 2024/03/11

REPOSITORIES: GEO

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The impact of substrate stiffness on morphological, transcriptional and functional aspects in RPE [RNA-Seq]

Project description:To investigate the influence of stiffness as a major Bruch's membrane characteristic on the RPE transcriptome and morphology. ARPE-19 cells were plated on soft or stiff polyacrylamide gels (PA gels) or standard tissue culture plastic (TCP). We then performed gene expression profiling analysis using data obtained from Next-generation sequencing of small RNAs of ARPE-19 cells cultured on 3 different biomechanical conditions.

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The impact of substrate stiffness on morphological, transcriptional and functional aspects in RPE

Project description:This SuperSeries is composed of the SubSeries listed below.

2024-03-11 | GSE225642 | GEO

Gene expression signitures in ARPE-19 cells treated with or without TGF-β1

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Project description:This study compared the gene expression change of ARPE-19 cells before and after adriamycin treatment ARPE-19 cells were treated with 1Î¼M adriamycin for 24 h compared with control

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Project description:We used microarrays to evaluate the effect of SRPIN803 on gene expression in ARPE-19 cells. ARPE-19 cells were treated with SRPIN803 (10 uM) or the negative control (0.1% DMSO) for 4 hours for Total RNA isolation and hybridization on Microarray.

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