Similar Datasets
Project description:Characterization of the effect of perturbation of sympathetic nervous system (SNS) on skeletal muscle rhythmic transcriptome.
2024-02-01 | GSE225851 | GEO
Project description:Malnutrition and low muscle mass (sarcopenia) are common problems in patients with cancer. However, a low muscle mass is associated with negative clinical outcomes in patients with cancer. Therefore, it is very important to maintain muscle mass in this population. This study aims to investigate the effect of an oral nutritional supplement on skeletal muscle mass during anti-cancer treatment.
| 2256 | ecrin-mdr-crc
Project description:The circadian regulation of transcriptional processes has a broad impact on cell metabolism. Here, we compared the diurnal transcriptome of human skeletal muscle conducted on serial muscle biopsies in vivo with profiles of human skeletal myotubes synchronized in vitro. Extensive rhythmic transcription was observed in human skeletal muscle in comparison to in vitro cell culture. However, nearly half of the in vivo rhythmicity was lost at the mRNA accumulation level. siRNA-mediated clock disruption in primary myotubes significantly affected the expression of ~8% of all genes, with impact on glucose homeostasis and lipid metabolism. Genes involved in GLUT4 expression, translocation and recycling were negatively affected, whereas lipid metabolic genes were altered to promote activation of lipid utilization. Moreover, basal and insulin stimulated glucose uptake were significantly reduced upon CLOCK depletion. Altogether, our findings suggest an essential role for cell-autonomous circadian clocks in coordinating muscle glucose homeostasis and lipid metabolism in humans.
2018-04-16 | GSE108539 | GEO
Project description:The goal of this study was to define the binding sites of the core circadian factors, BMAL1 and CLOCK across the genome in adult mouse skeletal muscle. We found that mitochondrial inner-membrane genes were among the most enriched rhythmic genes via gene ontology analysis, and ChIP-Seq analysis corroborated the role of Clock and Bmal1 in targeting promoters of mitochondrial inner-membrane genes.
2021-08-12 | GSE143334 | GEO
Project description:Effect of Sidt2 deficiency on skeletal muscle
| PRJNA450342 | ENA
Project description:Transcriptomic analyses reveal rhythmic and CLOCK-driven pathways in human skeletal muscle
| PRJNA427586 | ENA
Project description:In utero undernutrition is associated with obesity and insulin resistance, although its effect on skeletal muscle remains poorly defined. We report that, in mice, adult offspring from undernourished dams have decreased energy expenditure, decreased skeletal muscle mitochondrial content, and altered energetics in isolated mitochondria and permeabilized muscle fibers. Strikingly, when these mice are put on a 40% calorie restricted diet they lose half as much weight as calorie restricted controls. Our results reveal for the first time that in utero undernutrition alters metabolic physiology having a profound effect on skeletal muscle energetics and response to calorie restriction in adulthood.
2014-08-01 | GSE53520 | GEO
Project description:To study the effect of Sidt2 protein deficiency on skeletal muscle, we developed skeletal musclespecific Sidt2 gene knockout mouse model using Cre under the control of the MCK promoter We used microarrays to detail the changes of gene expression.
2018-04-17 | GSE113167 | GEO
Project description:In utero undernutrition is associated with obesity and insulin resistance, although its effect on skeletal muscle remains poorly defined. We report that, in mice, adult offspring from undernourished dams have decreased energy expenditure, decreased skeletal muscle mitochondrial content, and altered energetics in isolated mitochondria and permeabilized muscle fibers. Strikingly, when these mice are put on a 40% calorie restricted diet they lose half as much weight as calorie restricted controls. Our results reveal for the first time that in utero undernutrition alters metabolic physiology having a profound effect on skeletal muscle energetics and response to calorie restriction in adulthood. We have used a mouse model of low birth weight generated through 50% food restriction of mouse dams during the third week of gestation. We have studied in utero food restricted offspring and control offspring that were not food restricted in utero in both the ad libitum and calorie restricted states. Gene expression profiling was performed on tibialis anterior muscle from 8 mice per group, pooled in pairs.
2014-08-01 | E-GEOD-53520 | biostudies-arrayexpress
Project description:In this experiment we have analyzed the effect of TGFbeta-1 incubation (3h of incubation time) on the gene expression profiles of human primary cultured skeletal muscle cells, both in growing myoblasts or in differentiated muscle fibres (after 10-days of differentiation), obtained from skeletal muscle biopsies from a 2-months old healthy donor and a 5-years old DMD (Duchenne Muscular Dystrophy) patient. All human skeletal muscle biopsies were obtained by surgery from calf muscles with informed consent and approval of the Human Use Committee of Vall d'Hebron University Hospital (Barcelona, Spain). Human myoblasts were cultured on 6 cm plates until reached 70-80% of confluence and then, were incubated for 3h in the presence or in absence of TGF-?1 (2.5 ng/ml). Skeletal muscle fibres were obtained by growing human myoblasts on 6 cm plates until reached 70-80% of confluence and then, cells were induced to differentiate by deprivation of growth factors in the culture medium. After 10 days of differentiation, skeletal muscle fibers were incubated for 3h in the presence or in absence of TGFbeta-1 (2.5 ng/ml). In all cases, immediately after TGFbeta-1 treatment the RNA was extracted and used for Affymetrix microarrays analysis using the U133 2.0 microarray genechips.
2016-08-07 | E-MEXP-2937 | biostudies-arrayexpress