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A common set of genes responding to florigenic and photoperiodic induction at the shoot apical meristem of rice

ABSTRACT: Flowering of rice is triggered by transcriptional reprogramming at the shoot apical meristem (SAM) mediated by florigenic proteins produced in leaves in response to changes in the photoperiod. Florigens are more rapidly expressed under short day (SD) and include the HEADING DATE 3a (Hd3a) and RICE FLOWERING LOCUS T1 (RFT1) Phosphatidyl Ethanolamine Binding Proteins (PEBP). Hd3a and RFT1 are largely redundant at converting the SAM into an inflorescence, but whether they activate the same target genes and convey all photoperiodic information that modifies gene expression at the SAM is currently unclear. We uncoupled the contribution of Hd3a, RFT1 and SD to transcriptome reprogramming at the SAM, by RNA-sequencing of dex-inducible over-expressors of single florigens and wild type plants exposed to photoperiodic induction. Fifteen highly differentially expressed genes common to Hd3a, RFT1 and SD were retrieved, ten of which still uncharacterized. Detailed functional studies on some candidates revealed a role for LOC_Os04g13150 in determining tiller angle and bract development and the gene was renamed BROADER TILLER ANGLE 1 (BRT1). We identified a core set of genes common to florigenic and photoperiodic induction and defined the function of a novel florigen target controlling tiller angle.

ORGANISM(S): Oryza sativa

PROVIDER: GSE226057 | GEO | 2024/02/01

REPOSITORIES: GEO

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Oryza sativa

Project description:Hd3a, RFT1 and Ehd1 integrate photoperiodic and drought stress signals to delay the floral transition in rice

| PRJNA314526 | ENA

Microarray comparative analysis of wild-type and hd1-3 mutant rice

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Role of bHLH93 in controlling flowering time in Arabidopsis

Project description:Flowering in plants is a very dynamic and synchronized process where various cues including age, day-length, temperature and endogenous hormones fine-tune the timing of flowering for reproductive success. Arabidopsis thaliana is a facultative long day plant where long-day (LD) photoperiod promotes flowering. Arabidopsis still flowers under short-day (SD) conditions, albeit much later than LD conditions. Although, factors regulating the photoperiodic LD pathway have been extensively investigated, the SD pathway is much less understood. Here we identified a critical transcription factor called bHLH93 (basic Helix-Loop-Helix 93) that is essential to induce flowering specifically under SD conditions in Arabidopsis. bhlh93 mutants do not flower from primary meristem under SD conditions, but flowers similar to wild type under LD conditions. The late flowering phenotype is rescued by exogenous application of GA, suggesting that bHLH93 acts upstream of GA pathway to promote flowering. Double mutant studies showed that bhlh93 is epistatic to phyB and soc1 genes under SD conditions. bHLH93 is expressed at the meristematic regions and its expression peaks at 8 hours after dawn under SD conditions. As expected, the bHLH93 is localized in the nucleus. Taken together, these data suggest that bHLH93 is a key transcription factor necessary for Arabidopsis thaliana to evolve as a facultative plant.

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