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Genome-wide CRISPR loss-of-function screen for α-amanitin

ABSTRACT: The “death cap”, Amanita phalloides, is the world’s most poisonous mushroom, responsible for 90% of mushroom-related fatalities. The most fatal component of the death cap is α-amanitin(AMA). Despite its lethal effect, the exact mechanisms of how α-amanitin poisons humans remain unclear, leading to no specific antidote available for treatment. Here, we performed a genome-wide CRISPR loss-of-function screen to identify genes and pathways involved in AMA cytotoxicity.

ORGANISM(S): Homo sapiens

PROVIDER: GSE226447 | GEO | 2023/03/07

REPOSITORIES: GEO

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Characterization of protein dynamics after alpha-amanitin exposure in hepatoma cells

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