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ANXA1 promotes cell proliferation and drug-resistance in B cell acute lymphoblastic leukemia

ABSTRACT: ANXA1 promoted cell proliferation, suppressed cell apoptosis induced cell cycle arrest in B-ALL cells by CCK8, Flow cytometry and RNA-seq Sequencing analysis. The in vivo results indicated that down-regulation of ANXA1 in B-ALL cells could significantly reduce leukemic cell burden. We then performed gene expression profiling using data obtained from RNA-seq of B-ALL cells with the ANXA1 gene down-regulated in CCRF-SB.

ORGANISM(S): Homo sapiens

PROVIDER: GSE226449 | GEO | 2024/02/01

REPOSITORIES: GEO

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miRNA expression data from MCF-7 cells, overexpressing ANXA1

Project description:Annexin 1 (ANXA1), an endogenous anti-inflammatory protein which modulates cellular processes such as proliferation, differentiation and apoptosis has been implicated in cancer initiation and progression. ANXA1 was previously shown to be regulated by hsa-miR-196a and promoted cell proliferation and anchorarge-dependent growth and suppressed apoptosis. However, whether ANXA1 itself regulates miRNA expression is unknown. Therefore, in this study, we investigated the regulation of miRNA by ANXA1 in breast cancer cells. Using microarray technology, 12 miRNAs were found to be significantly and consistently downregulated in MCF-7 cells (MCF-V5) overexpressing ANXA1 overexpressing MCF-7 cells (MCF-V5). Hsa-miR-26b* and hsa-miR-562 were chosen for further investigation.The data suggest that miR-26b* and miR-562 may play a role in ANXA1-induced migration and possibly angiogenesis by targeting NFKB and point towards a potential therapeutic target for breast cancer. Breast cancer MCF-7 cells (MCF-V5) overexpressing ANXA1 were cultured for RNA extraction and hybridization on Affymetrix miRNA microarrays. These were compared against the control, which were MCF-7 cells (MCF-EV) carrying an empty expression vector. Expression analyses were carried out in triplicates

2015-01-05 | E-GEOD-54439 | biostudies-arrayexpress

miRNA expression data from MCF-7 cells, overexpressing ANXA1

2015-01-05 | GSE54439 | GEO

AnxA1 inhibits pathological bone resorption

Project description:AnxA1 inhibits pathological bone resorption

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ANXA1 promotes tumor immune evasion by binding PARP1 and upregulating the transcription of PD-L1 in the multiple cancers

Project description:To search proteins that interact with ANXA1, ANXA1 interactors were coimmunoprecipitated with anti-ANXA1 antibody from MB231 cell extracts, separated on SDS-PAGE and stained with Coomassie blue , and non-immune IgG antibody instead of anti-ANXA1 antibody served as control. All bands were excised, and subjected to in gel trypsin digestion and liquid chromatography and high-throughput mass spectrometry (LC-MS/MS) analysis.As a result, a total of 489 proteins including PARP1 were identified .

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Glucocorticoids induced transcriptional regulation in acute lymphoblastic leukemia

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